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Recombinant human NTRK2 /TRKB Protein, His Tag, 1mg  

Recombinant human NTRK2 /TRKB Protein, His Tag, 1mg

Recombinant Human NTRK2 /TRKB Protein Cys 32 - His 430, produced in human 293 cells (HEK293), His Tag

Synonym
recombinant human protein, NTRK2, TRKB, GP145-TrkB

More details

NT2-H5228-1K

Availability: within 7 days

1 500,00 €

Background
Neurotrophic tyrosine kinase receptor type 2 (NTRK2) also known as BDNF/NT-3 growth factors receptor, Tropomyosin-related kinase B (TRKB) and TrkB tyrosine kinase, which belongs to the protein kinase superfamily or Tyr protein kinase family. Insulin receptor subfamily. NTRK2 / TrkB contains two Ig-like C2-type (immunoglobulin-like) domains, two LRR (leucine-rich) repeats, one LRRCT domain, one LRRNT domain, one protein kinase domain. NTRK2 / Trk-B is expressed in the central and peripheral nervous system. The catalytic activity of NTRK2 is “ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate”. NTRK2 / TrkB involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity.

Source
Recombinant Human NTRK2 /TRKB Protein (rhNTRK2) Cys 32 - His 430 (Accession # AAH31835) was produced in human 293 cells (HEK293).

Molecular Characterization
rhNTRK2, fused with 6×His tag at the C-terminus, has a calculated MW of 45 kDa. The predicted N-terminus is Cys 32. DTT-reduced Protein migrates as 60-90 kDa due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhNTRK2 by the LAL method.

Purity
>95% purity as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
N/A

References

(1) Allen S.J., et al., 1994, Neuroscience 60:825-834.
(2) Stoilov P., et al., 2002, Biochem. Biophys. Res. Commun. 290:1054-1065.
(3) Meakin S.O., et al., 1999, J. Biol. Chem. 274:9861-9870.
(4) Yeo G.S., et al., 2004, Nat. Neurosci. 7:1187-1189.