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Recombinant human CEACAM1 /CD66a /BGP1 Protein, 1mg  

Recombinant human CEACAM1 /CD66a /BGP1 Protein, 1mg

Recombinant Human CEACAM1 /CD66a /BGP1 Protein (rhCEACAM1 /CD66a) Gln 35 - Gly 428 was produced in human 293 cells (HEK293)

Synonym recombinant, human, protein CEACAM1, CD66a, BGP, BGP1, BGPI

More details

CE1-H5220-1K

Availability: within 7 days

1 500,00 €

Background
Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1), also known as Biliary glycoprotein 1 (BGP1), CD66a, which belongs to the immunoglobulin superfamily or CEA family. CEACAM1 /CD66a contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CEACAM1 /CD66a was described as an adhesion molecule mediating cell adhesion via both homophilic and heterophilic manners, and was detected on leukocytes, epithelia, and endothelia. Studies have revealed that CEACAM1 / BGP-1 performs actions in multiple cellular processes including tissue differentiation, angiogenesis, apoptosis, metastasis, as well as the modulation of innate and adaptive immune responses.

Source
Recombinant Human CEACAM1 /CD66a /BGP1 Protein (rhCEACAM1 /CD66a) Gln 35 - Gly 428 (Accession # AAH14473) was produced in human 293 cells (HEK293).

Molecular Characterization
rhCEACAM1 /CD66a, fused with 6×His tag at the C-terminus, has a calculated MW of 44.2 kDa. The predicted N-terminus is Gln 35. DTT-reduced Protein migrates as 60-130 kDa due to different glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCEACAM1 /CD66a by the LAL method.

Purity
>92% purity as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Kuroki M., et al., 1991, Biochem. Biophys. Res. Commun. 176:578-585.
(2) Watt S.M., et al., 1994, Blood 84:200-210.
(3) Chen R., et al., 2009, J. Proteome Res. 8:651-661.
(4) Hoek KS., et al., 2008, Pigment Cell Melanoma Res. 21 (6): 665–76.