Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant human NTRK2 /TRKB Protein, Fc Tag, 1mg  

Recombinant human NTRK2 /TRKB Protein, Fc Tag, 1mg

Recombinant Human NTRK2 /TRKB Protein, Cys 32 - His 430 was produced in human 293 cells (HEK293), Fc Tag

Synonym
recombinant human protein, NTRK2, TRKB, GP145-TrkB

More details

NT2-H5254-1K

Availability: within 7 days

1 440,00 €

Background
Neurotrophic tyrosine kinase receptor type 2 (NTRK2) also known as BDNF/NT-3 growth factors receptor, Tropomyosin-related kinase B (TRKB) and TrkB tyrosine kinase, which belongs to the protein kinase superfamily or Tyr protein kinase family. Insulin receptor subfamily. NTRK2 / TrkB contains two Ig-like C2-type (immunoglobulin-like) domains, two LRR (leucine-rich) repeats, one LRRCT domain, one LRRNT domain, one protein kinase domain. NTRK2 / Trk-B is expressed in the central and peripheral nervous system. The catalytic activity of NTRK2 is “ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate”. NTRK2 / TrkB involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity.

Source
Recombinant Human NTRK2 /TRKB Protein, with C-Fc Tag (rhNTRK2 Fc Chimera) Cys 32 - His 430 (Accession # AAH31835) was produced in human 293 cells (HEK293).

Molecular Characterization
rhNTRK2 Fc Chimera, fused with Fc fragment of human IgG1 at the C-terminus, has a calculated MW of 70.4 kDa. The predicted N-terminus is Cys 32. DTT-reduced Protein migrates as 95-120 kDa due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhNTRK2 Fc Chimera by the LAL method.

Purity
>98% purity as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
N/A


References

(1) Allen S.J., et al., 1994, Neuroscience 60:825-834.
(2) Stoilov P., et al., 2002, Biochem. Biophys. Res. Commun. 290:1054-1065.
(3) Meakin S.O., et al., 1999, J. Biol. Chem. 274:9861-9870.
(4) Yeo G.S., et al., 2004, Nat. Neurosci. 7:1187-1189.