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Recombinant human ALK-3/BMPR1A Protein, 1 mg  

Recombinant human ALK-3/BMPR1A Protein, 1 mg

Recombinant bone morphogenetic protein receptor,type IA Fc Chimera (rhBMPR1A-Fc or rhALK3-Fc), Gln24- Arg152 was expressed in HEK 293

Synonym: recombinant, human, protein BMPR-1A, ALK-3, BMPR1A,ACVRLK3, ALK3, bone morphogenetic protein receptor type IA

More details

AL3-H5259-1K

Availability: within 7 days

990,00 €

Background
The bone morphogenetic protein receptor, type IA also known as BMPR1A or ALK3 is a protein which in humans is encoded by the BMPR1A gene. BMPR1A has also been designated as CD292 (cluster of differentiation 290). The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR1A (this protein) and BMPR1B and the type II receptor BMPR2. These receptors are also closely related to the activin receptors, ACVR1 and ACVR2[1]. Alk3-mediated BMP signaling in AV endocardial/mesenchymal cells plays a central role during cushion morphogenesis[2].

Source
Recombinant Bone morphogenetic protein receptor,type IA Fc Chimera (rhBMPR1A-Fc or rhALK3-Fc), Gln24- Arg152(Accession # CAA80257) was expressed in HEK 293.

Molecular Characterization
rhALK3-Fc, fused with Fc fragment of human IgG1 at C-terminus, and has a calculated MW of 42 kDa. The predicted N-terminal is Gln24. DTT-reduced protein monomer migrates as 56 kDa resulting from glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhALK3-Fc by the LAL method.

Purity
>98% purity as determined by SDS-PAGE of reduced rhALK3-Fc.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).



References

(1) Mishina Y, Starbuck MW , et al.(2004). J. Biol. Chem. 279 (26): 27560–6
(2) Song L, Fässler R, et al. (2007). Dev Biol. 301(1):276-86