Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Mouse EGFR /ErbB1 Protein,  His Tag,1mg  

Recombinant Mouse EGFR /ErbB1 Protein, His Tag,1mg

Recombinant Mouse EGF R Protein (Mouse EGF R, His Tag) Leu 25 - Ser 647 was produced in human 293 cells (HEK293)

Synonym
recombinant, mouse, protein, EGFR, ERBB, ERBB1, HER1, PIG61, mENA

More details

EGR-M5224-1K

Availability: within 7 days

1 980,00 €

Background
The epidermal growth factor receptor (EGFR; ErbB-1; HER1) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands.[1] The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.[2]

Source
Mouse EGF R, His Tag (EGR-M5224) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 647 (Accession # Q01279-1).
Predicted N-terminus: Leu 25
Request for sequence

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 71.2 kDa. The protein migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per 1 μg of the mouse EGFRG by the LAL method.

Purity
>95% as determined by SDS-PAGE. All lots are greater than 95% pure.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized mouse EGF at 10 μg/ml can bind mouse EGFR with a linear range of 2.5–1000 ng/ml.

References

(1) Herbst RS, 2004, Int. J. Radiat. Oncol. Biol. Phys. 59 (2 Suppl): 21–6.
(2) Zhang H, et al.,2007, Journal of Clinical Investigation 117(8):2051-2058.