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Recombinant Human ADAM12 Protein, 50µg  

Recombinant Human ADAM12 Protein, 50µg

Recombinant Human ADAM12 Protein (rhADAM12) Arg 29 - Asp 513 was produced in human 293 cells (HEK293)

Synonym
recombinant, human, protein ADAM12, MCMP, MLTN, MLTNA, MCMPMltna, Meltrin Alpha

More details

AD2-H5228-050

Availability: within 7 days

312,00 €

Background
A Disintegrin And Metalloprotease 12 (ADAM12) is also known as Meltrin-alpha and MCMP, is a member of the ADAM and MDC (Metalloprotease, Disintegrin, Cysteine-rich) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving in diverse processes such as asthma, development, angiogenesis and cancer through their activities in cell adhesion/fusion, membrane protein shedding, and signal transduction. Over 30 members have been identified and about half of them are active metalloproteases such as ADAM8, 9, 10, 12 and 17/TACE. ADAM12 has four Isoform, Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. ADAM12 Involved in skeletal muscle regeneration, specifically at the onset of cell fusion and also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.

Source
Recombinant Human ADAM12 Protein (rhADAM12) Arg 29 - Asp 513 (Accession # AAH60804.1) was produced in human 293 cells (HEK293).

Molecular Characterization
rh ADAM12 is fused with 6 ×his tag at the C terminus, and has a calculated MW of 55.2 kDa (Arg 29 - Asp 513, Propeptide 20.1 kDa + mature form 34.5 kDa). The rh ADAM12 (Arg 29 - Asp 513) was cleaved into the propeptide and the mature form. DTT-reduced Protein migrates as 65 kDa, 46 kDa and 23 kDa in SDS-PAGE due to glycosylation, corresponding to the proprotein form, the mature form and the propeptide respectively.

Endotoxin
Less than 1.0 EU per μg of the rhADAM12 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Refer to data sheet

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
N/A

References

(1) Iba K., et al., 2000, J. Cell Biol. 149:1143-1156.
(2) Abram C.L., et al., 2003, J. Biol. Chem. 278:16844-16851.
(3) Bartholin L., et al., 2005, Biol. Cell 97:577-588.
(4) Bourd-Boittin K., 2008, J. Biol. Chem. 283:26000-26009.