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Recombinant Human PLGF/PIGF2/PGF Protein, His tag, 500µg  

Recombinant Human PLGF/PIGF2/PGF Protein, His tag, 500µg

Recombinant Human PLGF / PGF Protein (rhPGF) Leu 19 - Arg 170 was produced in human 293 cells (HEK293), His Tag

Synonym
Recombinant Human Protein, PGF, PLGF, PlGF2, PlGF, PGFL, SHGC-10760

More details

PGF-H5229-500

Availability: within 7 days

1 600,00 €

Background
Placental growth factor (PGF) also known as vascular endothelial growth factor-related protein, PLGF and PlGF2, is a member of the VEGF (vascular endothelial growth factor) sub-family - a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. The main source of PGF during pregnancy is the placental trophoblast. PGF is also expressed in many other tissues, including the villous trophoblast. PGF is actived in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. PlGF2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth.

Source
Recombinant Human PlGF Protein (rhPGF) Leu 19 - Arg 170 (Accession # NP_002623.2) was produced in human 293 cells (HEK293).

Molecular Characterization
rhPGF, fused with 6×His tag at the C-terminus, has a calculated MW of 20.2 kDa. The predicted N-terminus is Leu 19. DTT-reduced Protein migrates as 28-33 kDa due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhPGF by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 100 mM acetic acid, pH2-3. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity-ELISA
Immobilized Rhesus macaque VEGF R1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. VE1-R5257) at 5 μg/mL (100 μL/well) can bind Human PLGF, His Tag (Cat. No. PGF-H5229) with a linear range of 5-40 ng/mL (QC tested).
Immobilized Human PLGF, His Tag (Cat. No. PGF-H5229) at 1 μg/mL (100 μL/well) can bind Biotinylated Human VEGF R1, His,Avitag (Cat. No. VE1-H82E3) with a linear range of 2-20 ng/mL (Routinely tested).

References

(1) Maglione D., et al., 1993, Oncogene 8 (4): 925–31.
(2) Khalil A., et al., 2008, PLoS ONE 3 (7): e2766.
(3) Khurana R., et al., 2005, Circulation 111 (21): 2828–2836.
(4) Rolny C., et al., 2011, Cancer Cell 19:31-44.