Products

Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Rhesus Macaque VEGFR2 /KDR Protein, His Tag, 2x500ug  

Recombinant Rhesus Macaque VEGFR2 /KDR Protein, His Tag, 2x500ug

Recombinant Rhesus Macaque VEGFR2 /KDR Protein, Ala 20 - Glu 764, produced in human HEK293 cells, His Tag

Synonym: recombinant rhesus macaque protein, KDR,CD309,FLK1,VEGFR,VEGFR2, kinase insert domain receptor

More details

VE2-C52H3-1K

Availability: within 7 days

1 800,00 €

Background
Kinase insert domain receptor (KDR) also known as CD309, FLK1, VEGFR, VEGFR2, and is one of the subtypes of VEGFR. VEGF receptors are receptors for vascular endothelial growth factor (VEGF). There are three main subtypes of VEGFR, numbered 1, 2 and 3. The VEGF receptors have an extracellular portion consisting of 7 immunoglobulin-like domains, a single transmembrane spanning region and an intracellular portion containing a split tyrosine-kinase domain. VEGF-A binds to VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1). VEGFR-2 appears to mediate almost all of the known cellular responses to VEGF.The function of VEGFR-1 is less well defined, although it is thought to modulate VEGFR-2 signaling. Another function of VEGFR-1 may be to act as a dummy/decoy receptor, sequestering VEGF from VEGFR-2 binding (this appears to be particularly important during vasculogenesis in the embryo).[1-3] In addition, VEGFR2 is able to interact with HIV-1 extracellular Tat protein upon VEGF activation, and seems to enhance angiogenesis in Kaposi\'s sarcoma lesions.

Source
Recombinant Rhesus macaque VEGF R2, His Tag (VE2-C52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Glu 764 (Accession # F7E313).
Predicted N-terminus: Ala 20

Molecular Characterization
Rhesus Macaque VEGFR2 / KDR is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 85.2 kDa. The predicted N-terminus is Ala 20. DTT-reduced Protein migrates as 120-130 kDa in SDS-PAGE .

Endotoxin
Less than 1.0 EU per μg of the Rhesus Macaque VEGFR2 / KDR by the LAL method.

Purity
>97% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4C-8C); After reconstitution under sterile conditions for 3 months (-20C to -70C).

Bioactivity-ELISA
Immobilized ActiveMax® Human VEGF165, Tag Free (HPLC-verified) (Cat. No. VE5-H4210) at 2 μg/mL (100 μL/well) can bind Rhesus macaque VEGF R2, His Tag (Cat. No. VE2-C52H3) with a linear range of 4-60 ng/mL (QC tested).

References

(1) Holmes K,et al. (2007) . Cell Signal. 19 (10): 2003–2012.
(2) Stuttfeld E, et al. (2009). IUBMB Life 61 (9): 915–22.
(3) Fujita, N, et al. (2008). Biochemical and biophysical research communications 372 (2): 367–372.

The following products could also be interesting for you: