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Recombinant Human Azurocidin /AZU1 Protein, 1mg  

Recombinant Human Azurocidin /AZU1 Protein, 1mg

Recombinant Human Azurocidin / CAP37 / AZU1 Protein (rhAZU1 / HBP) Ile 27 - Pro 250 was produced in human 293 cells (HEK293), His Tag

Synonym: recombinant, human, protein, AZU1, Azurocidin, HBP, AZAMP, AZU

More details

AZ1-H5225-1K

Availability: within 7 days

1 440,00 €

Background
Azurocidin (AZU1) is also known as Heparin-binding protein (HBP), Cationic antimicrobial protein CAP37, is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. which belongs to the peptidase S1 family and elastase subfamily. AZU1 / HBP contains 1 peptidase S1 domain. AZU1 binds heparin. The cytotoxic action of AZU1 is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. AZU1 may play a role in mediating recruitment of monocytes in the second wave of inflammation.

Source
Recombinant Human Azurocidin, His Tag (AZ1-H5225) is expressed from human 293 cells (HEK293). It contains AA Ile 27 - Pro 250 (Accession # AAH69495).
Predicted N-terminus: Ile 27

Molecular Characterization
rhAZU1 / HBP, fused with 6×His tag at the C-terminus, has a calculated MW of 25 kDa. The predicted N-terminus is Ile 27. DTT-reduced Protein migrates as 32-40 kDa due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhAZU1 / HBP by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Contact us for customized product format or formulation.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4C-8C); After reconstitution under sterile conditions for 1 month (4C-8C) or 3 months (-20C to -70C).

References

(1) Wasiluk K.R., et al., 1991, Infect. Immun. 59:4193-4200.
(2) Miyasaki K.T., 1992, Infect. Immun. 60:4973-4975.
(3) Linder A., 2009, Clin. Infect. Dis. 49 (7): 1044–50.