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Recombinant Human TNFSF4 /OX40L Protein, Fc Tag, 1mg  

Recombinant Human TNFSF4 /OX40L Protein, Fc Tag, 1mg

Recombinant Human TNFSF4 /OX40L Protein,N-Fc Tag (rh TNFSF4 / OX40L Fc Chimera) Gln 51 - Leu 183 was produced in human 293 cells (HEK293)

Synonyms: recombinant human protein OX40L,TNFSF4,CD252

More details

OXL-H526x-1K

Availability: within 7 days

1 980,00 €

Background
Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L), TAX transcriptionally-activated glycoprotein 1 and CD252, which belongs to the tumor necrosis factor family. TNFSF4 is the ligand for CD134 and is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. Furthermore, similar to other TNF superfamily members, membrane-bound OX40 Ligand (TNFSF4) exists as a homotrimer. Human TNFSF4 shares 46% amino acid sequence identity with its mouse counterpart.

Source
Recombinant Human TNFSF4 /OX40L Protein,N-Fc Tag (rh TNFSF4 / OX40L Fc Chimera) Gln 51 - Leu 183 (Accession # NP_003317) was produced in human 293 cells (HEK293)

Molecular Characterization
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 42.4 kDa. The protein migrates as 53-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh TNFSF4 / OX40L Fc Chimera by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Please refer to product data sheet.

Bioactivity
Please refer to product data sheet.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4C-8C); After reconstitution under sterile conditions for 1 month (4C-8C) or 3 months (-20C to -70C). 

References

(1) Compaan D.M., et al., 2006, Structure 14:1321-1330.
(2) Maizels RM., et al., 2003, Nat. Rev. Immunol. 3 (9): 733–44.