Recombinant CLEC3B / Tetranectin Protein, His Tag, 100µg

Background
C-type lectin domain family 3 member B (CLEC3B) is also known as Tetranectin, Plasminogen kringle 4-binding protein, is a secreted homotrimer. CLEC3B contains 1 C-type lectin domain and mainly found in plasma. Tetranectin / CLEC3B binds to plasminogen and to isolated kringle 4. CLEC3B may be involved in the packaging of molecules destined for exocytosis. Decreased plasma Tetranectin or increased Tetranectin in stroma of cancers correlates with cancer progression and adverse prognosis.

Source
Recombinant Human CLEC3B /Tetranectin Protein (rhCLEC3B) Glu 22 - Val 202  was produced in human 293 cells (HEK293).

Molecular Characterization
rhCLEC3B is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 21 kDa. The predicted N-terminus is Glu 22. The reducing (R) protein migrates as 22 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 0.1 EU per μg of the rhCLEC3B by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
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Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

Bioactivity
n/a

References

(1) Fuhlendorff J., et al., 1987, Biochemistry 26:6757-6764.
(2) Jaquinod M., et al., 1999, Biol. Chem. 380:1307-1314.