Products

Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Human Mesothelin / MSLN Protein, His-Tag, 100µg  

Recombinant Human Mesothelin / MSLN Protein, His-Tag, 100µg

Recombinant Human Mesothelin / MSLN (296-580) Protein, expressed from human HEK293 cells, His-Tag

Synonym
recombinant, human, protein, MSLN,Mesothelin,MPF

More details

MSN-H5223-100

Availability: within 7 days

378,00 €

Background
Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.

Source
Human Mesothelin (296-580), His Tag (MSN-H5223) is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272).
Predicted N-terminus: Glu 296

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 33.0 kDa. The protein migrates as
32-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4) with Trehalose as protectant.
Contact us for customized product form or formulation

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Bioactivity-ELISA
Immobilized Human Mesothelin (296-580), His Tag (Cat. No. MSN-H5223) at 0.5 μg/mL (100 μL/well) can bind Anti-Human MSLN MAb with a linear range of 0.1-2 ng/mL (QC tested).

Bioactivity-SPR
Anti-Human MSLN MAb (chimeric mouse-human IgG1) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human Mesothelin (296-580), His Tag (Cat. No. MSN-H5223) with an affinity constant of 0.518 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

References

(1) Chang K., et al., 1996, Proc. Natl. Acad. Sci. U.S.A. 93:136-140.
(2) Yamaguchi N., et al., 1994, J. Biol. Chem. 269:805-808.
(3) Rump A., et al., 2004, J. Biol. Chem. 279:9190-9198.
(4) Ma J., et al., 2012, J. Biol. Chem. 287:33123-33131.