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Recombinant Human Mesothelin / MSLN Protein, His-Tag, 2 x 500µg  

Recombinant Human Mesothelin / MSLN Protein, His-Tag, 2 x 500µg

Recombinant Human Mesothelin / MSLN (296-580) Protein, expressed from human HEK293 cells, His-Tag

recombinant, human, protein, MSLN,Mesothelin,MPF

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Availability: within 7 days

1 980,00 €

Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.

Human Mesothelin (296-580), His Tag (MSN-H5223) is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272).
Predicted N-terminus: Glu 296

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 33.0 kDa. The protein migrates as
32-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Less than 1.0 EU per μg by the LAL method.

>95% as determined by SDS-PAGE.

Lyophilized from 0.22µm filtered solution in PBS (pH 7.4) with Trehalose as protectant.
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See Certificate of Analysis for reconstitution instructions and specific concentrations.

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Immobilized Human Mesothelin (296-580), His Tag (Cat. No. MSN-H5223) at 0.5 μg/mL (100 μL/well) can bind Anti-Human MSLN MAb with a linear range of 0.1-2 ng/mL (QC tested).

Anti-Human MSLN MAb (chimeric mouse-human IgG1) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human Mesothelin (296-580), His Tag (Cat. No. MSN-H5223) with an affinity constant of 0.518 nM as determined in a SPR assay (Biacore T200) (Routinely tested).


(1) Chang K., et al., 1996, Proc. Natl. Acad. Sci. U.S.A. 93:136-140.
(2) Yamaguchi N., et al., 1994, J. Biol. Chem. 269:805-808.
(3) Rump A., et al., 2004, J. Biol. Chem. 279:9190-9198.
(4) Ma J., et al., 2012, J. Biol. Chem. 287:33123-33131.