Recombinant Human Mesothelin / MSLN Protein, Fc Tag, 1mg

Background
Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.

Source
Recombinant Human MSLN /Mesothelin (aa 296-580) Protein, With N-Fc Tag (rh MSLN / Mesothelin (aa 296-580) Fc Chimera) Glu 296 - Gly 580 (Accession # AAH09272) was produced in human 293 cells

Molecular Characterization
rh MSLN / Mesothelin (aa 296-580) Fc Chimera, fused with Fc fragment of human IgG1 at the N-terminus, has a calculated MW of 59.6 kDa. The predicted N-terminus is Glu 296. As a result of glycosylation, DTT-reduced Protein migrates as 60-68 kDa and non-reduced protein migrates as 120-130 kDa.

Endotoxin
Less than 1.0 EU per μg of the rh MSLN / Mesothelin (aa 296-580) Fc Chimera by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Immobilized Mouse LDL R, His Tag (Cat. No. LDR-M52H8) at 10 μg/mL (100 μL/well) can bind Biotinylated Mouse PCSK9, His Tag (Cat. No. PC9-M82E1) with a linear range of 2-16 ng/mL (QC tested).

References

(1) Chang K., et al., 1996, Proc. Natl. Acad. Sci. U.S.A. 93:136-140.
(2) Yamaguchi N., et al., 1994, J. Biol. Chem. 269:805-808.
(3) Rump A., et al., 2004, J. Biol. Chem. 279:9190-9198.
(4) Ma J., et al., 2012, J. Biol. Chem. 287:33123-33131.