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Human Carbonic Anhydrase IX / CA9 (138-414) Protein, His Tag, 50 µg  

Human Carbonic Anhydrase IX / CA9 (138-414) Protein, His Tag, 50 µg

Recombinant Human Carbonic Anhydrase IX / CA9 (138-414) Protein, Gln 138 - Asp 414 was produced in human 293 cells (HEK293), His Tag

Synonym: Recombinant human protein,CAIX,CA9,CA-IX,G250,MN,P54/58N,pMW1

More details

CA9-H5220-50

Availability: within 7 days

420,00 €

Background
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes. CAs form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. The active site of most carbonic anhydrases contains a zinc ion. There are at least five distinct CA families (α, β, γ, δ and ε). Carbonic anhydrase 9 (CA9 / CAIX) is also known as Membrane antigen MN (MN), Renal cell carcinoma-associated antigen G250, which belongs to the alpha-carbonic anhydrase family. CA9 / CAIX with an optimal activity at pH 6.49. Reversible hydration of carbon dioxide. CA IX participates in pH regulation. CA9 may be involved in the control of cell proliferation and transformation. CA-IX appears to be a novel specific biomarker for a cervical neoplasia.

Source
Recombinant Human Carbonic Anhydrase IX / CA9 (138-414) Protein (Human Carbonic Anhydrase IX (138-414), His Tag) Gln 138 - Asp 414 (Accession # NP_001207.2) was produced in human 293 cells (HEK293).

Molecular Characterization
Human Carbonic Anhydrase IX (138-414), His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 30.9 kDa. The predicted N-terminus is Gln 138. The reducing (R) protein migrates as 36 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Human Carbonic Anhydrase IX (138-414), His Tag by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

References

(1) Badger MR, Price GD, 1994, Annu. Rev. Plant Physiol. Plant Mol. Bio. 45: 369–392.
(2) Temperini C., et al., 2007, Bioorg. Med. Chem. Lett. 17:2210-2215.
(3) Hilvo M., et al., 2008, J. Biol. Chem. 283:27799-27809.
(4) Alterio V., et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106:16233-16238.