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Recombinant Human Carbonic Anhydrase IX / CA9 (138-414) Protein, His Tag, 50 µg  

Recombinant Human Carbonic Anhydrase IX / CA9 (138-414) Protein, His Tag, 50 µg

Recombinant Human Carbonic Anhydrase IX / CA9 (138-414) Protein, Gln 138 - Asp 414 was produced in human 293 cells (HEK293), His Tag

Synonym: Recombinant human protein,CAIX,CA9,CA-IX,G250,MN,P54/58N,pMW1

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Availability: within 7 days

420,00 €

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes. CAs form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. The active site of most carbonic anhydrases contains a zinc ion. There are at least five distinct CA families (α, β, γ, δ and ε). Carbonic anhydrase 9 (CA9 / CAIX) is also known as Membrane antigen MN (MN), Renal cell carcinoma-associated antigen G250, which belongs to the alpha-carbonic anhydrase family. CA9 / CAIX with an optimal activity at pH 6.49. Reversible hydration of carbon dioxide. CA IX participates in pH regulation. CA9 may be involved in the control of cell proliferation and transformation. CA-IX appears to be a novel specific biomarker for a cervical neoplasia.

Recombinant Human Carbonic Anhydrase IX (138-414), His Tag (CA9-H5220) is expressed from human 293 cells (HEK293). It contains AA Gln 138 - Asp 414 (Accession # NP_001207.2).
Predicted N-terminus: Gln 138

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 30.9 kDa. The protein migrates as 34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Less than 1.0 EU per μg of the Human Carbonic Anhydrase IX (138-414), His Tag by the LAL method.

>95% as determined by SDS-PAGE.

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).


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