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Recombinant Human CD3 epsilon & CD3 delta Heterodimer Protein, Fc Tag, 1mg  

Recombinant Human CD3 epsilon & CD3 delta Heterodimer Protein, Fc Tag, 1mg

Recombinant Human CD3E & CD3D heterodimer protein (CD3E: Asp 23 - Asp 126),  (CD3D: Phe 22 - Ala 105), produced in human 293 cells (HEK293), CD3E: His tag, Fc tag, CD3D: Flag tag, Fc tag (MALS verified)

Synonym: recombinant, human protein, CD3E & CD3D, CD3 delta & CD3 epsilon, CD3 delta / epsilon

More details

CDD-H52W0-1K

Availability: within 7 days

1 936,00 €

Background
T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Source
Recombinant Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (CDD-H52W0) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1 (CD3E) & P04234-1 (CD3D)).
Predicted N-terminus: Asp 23 (CD3E) & Phe 22 (CD3D)

Molecular Characterization
Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 39.2 kDa (CD3E) and 37.5 kDa (CD3D) . Subunit CD3E is fused with his-tagged human IgG1 Fc fragment at the C-terminus and subunit CD3D is fused with flag-tagged human IgG1 Fc fragment at the C-terminus. The reducing (R) heterodimer protein migrates as 40-55 kDa due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Human CD3E & CD3D Heterodimer Fc Chimera by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

Bioactivity
Please refer to product data sheet.

References

(1) Gold DP, et al., 1986, Nature 321 (6068): 431–4.
(2) Clevers HC, et al., 1988, Proc. Natl. Acad. Sci. U.S.A. 85 (21): 8156–60.
(3) Gil J, et al., 2011, J Clin Invest, 121(10):3872-6.
(4) Lu X, et al.,2011, Hematology, 16(3):185-9.

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