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Recombinant Human Mucin-1 / MUC-1 Protein, Llama IgG2b Fc Tag, low endotoxin, 1 mg (2x 500µg)  

Recombinant Human Mucin-1 / MUC-1 Protein, Llama IgG2b Fc Tag, low endotoxin, 1 mg (2x 500µg)

Recombinant Human Mucin-1 / MUC-1 Protein, AA Ser 33 - Gly 167, expressed from human 293 cells (HEK293), Llama IgG2b Fc Tag, low endotoxin

Synonym: Recombinant, human, protein, Mucin1, MUC1, CD227, EMA, H23AG, KL-6, MAM6, MUC-1/SEC, MUC-1/X, MUC1/ZD, PEM, PEMT, PUM, CA 15-3, Episialin

More details

MU1-H5255-1K

Availability: within 7 days

1 980,00 €

Background
Mucin-1 (MUC1) is also known as Tumor-associated epithelial membrane antigen (EMA), Polymorphic epithelial mucin (PEM), Peanut-reactive urinary mucin (PUM), PEMT, Krebs von den Lungen-6 (KL-6), CD antigen CD227, Episialin, H23AG. MUC1 is a glycoprotein with extensive O-linked glycosylation of its extracellular domain. Mucins line the apical surface of epithelial cells in the lungs, stomach, intestines, eyes and several other organs. Mucins protect the body from infection by pathogen binding to oligosaccharides in the extracellular domain, preventing the pathogen from reaching the cell surface. Except protective function by binding to pathogens, MUC1 also functions in a cell signaling capacity. Overexpression of MUC1 is often associated with colon, breast, ovarian, lung and pancreatic cancers.

Source
Recombinant Human Mucin-1, Llama IgG2b Fc Tag, low endotoxin (MU1-H5255) is expressed from human 293 cells (HEK293). It contains AA Ser 33 - Gly 167 (Accession # AAI20976).
Predicted N-terminus: Ser 33

Molecular Characterization
This protein carries a llama IgG2b Fc tag at the C-terminus.
The protein has a calculated MW of 42.4 kDa. The protein migrates as 50-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 0.01 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution

Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

References

(1)  Gendler SJ., et al., 1990, J. Biol. Chem. 265 (25): 15286–93.
(2)  Hollingsworth MA., et al., 2004, Nat. Rev. Cancer 4 (1): 45–60.
(3)  Moncada DM., et al., 2003, Trends Parasitol 19 (7): 305–311.
(4)  Singh PK., et al., 2006, Trends Cell Biol 16 (9): 467–476.
(5)  Lindén SK., et al., 2009, PLoS Pathog. 5 (10): e1000617.