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Recombinant Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag, 1 mg (2x 500µg)  

Recombinant Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag, 1 mg (2x 500µg)

Recombinant Cynomolgus CD3E&CD3G Heterodimer Protein,  AA Gln 22 - Asp 117 (CD3E) & Gln 23 - Thr 113 (CD3G), expressed from human 293 cells (HEK293), Fc Tag&Fc Tag

Synonym: Recombinant, cynomolgus, protein, CD3 epsilon & CD3 gamma, CD3E & CD3G

More details

CDG-C5255-1K

Availability: within 7 days

2 310,00 €

Background
T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Source
Recombinant Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (CDG-C5255) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (CD3E) & Gln 23 - Thr 113 (CD3G) (Accession # Q95LI5-1 (CD3E) & Q95LI7-1 (CD3G)).
Predicted N-terminus: Gln 22 (CD3E) & Gln 23 (CD3G)

Molecular Characterization
Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 37.3 kDa (CD3E) and 37.0 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus. The reducing (R) heterodimer protein migrates as 40-45 kDa due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution

Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity-ELISA
Immobilized Mouse Anti-Human CD3 (SP34-2) at 1 μg/mL (100 μL/well) can bind Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-C5255) with a linear range of 0.3-2 ng/mL (QC tested).

References

(1)  Gold DP, et al., 1986, Nature 321 (6068): 431–4.
(2)  Clevers HC, et al., 1988, Proc. Natl. Acad. Sci. U.S.A. 85 (21): 8156–60.
(3)  Gil J, et al., 2011, J Clin Invest, 121(10):3872-6.
(4)  Lu X, et al.,2011, Hematology, 16(3):185-9.