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Recombinant Human Integrin alpha 2 beta 1 (ITGA2&ITGB1) Heterodimer Protein, His Tag&Tag Free, 1 mg (2x 500µg)  

Recombinant Human Integrin alpha 2 beta 1 (ITGA2&ITGB1) Heterodimer Protein, His Tag&Tag Free, 1 mg (2x 500µg)

Recombinant Human Integrin alpha 2 beta 1 (ITGA2&ITGB1) Heterodimer Protein, AA Tyr 30 - Thr 1132 (ITGA2) & Gln 21 - Asp 728 (ITGB1), expressed from human 293 cells (HEK293), His Tag&Tag Free

Synonym: Recombinant, human, protein, Integrin alpha 2 beta 1, ITGA2 & ITGB1

More details

IT1-H52W6-1K

Availability: within 7 days

2 880,00 €

Background
Integrin alpha 2 beta 1 is one of twelve integrin family adhesion receptors that share the beta 1 subunit. It is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. Integrin ITGA2:ITGB1 acts as a receptor for Human rotavirus A and Human echoviruses 1 and 8. DGEA inhibited rotavirus binding to alpha2beta1 and infectivity. In a novel process, integrin-using viruses bind the alpha2 I domain of alpha2beta1 via DGE in VP4 and interact with alphaXbeta2 (via GPR) and alphaVbeta3 by using VP7 to facilitate cell entry and infection.

Source
Recombinant Human ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-H52W6) is expressed from human 293 cells (HEK293). It contains AA Tyr 30 - Thr 1132 (ITGA2) & Gln 21 - Asp 728 (ITGB1) (Accession # P17301-1 (ITGA2) & P05556-1 (ITGB1)).
Predicted N-terminus: Tyr 30 (ITGA2) & Gln 21 (ITGB1)

Molecular Characterization
Human ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA2 and ITGB1, has a calculated MW of 127.6 kDa (ITGA2) and 83.7 kDa (ITGB1). Subunit ITGA2 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 130 kDa (ITGA2) and 100-120 kDa (ITGB1) respectively due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, 2mM CaCl2, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution

Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity-ELISA
Immobilized Human Collagen I protein at 2 μg/mL (100 μL/well) can bind Human ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W6) with a linear range of 0.078-1.25 μg/mL (QC tested).

Immobilized Human Collagen I at 2 μg/mL (100 μL/well) can bind Human ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W6) with a linear range of 5-78 ng/mL (Routinely tested).

References

(1)  Graham K.L., et al. 2003. J. Virol. 77:9969-9978.
(2)  Bergelson J.M., et al. 1993. J. Virol. 67:6847-6852.
(3)  Takada, Y. et al. 2007. Genome Biol. 8:215.