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Recombinant Human R-Spondin 3 / RSPO3 (22-146) Protein, His Tag, 1 mg (4x 250µg)  

Recombinant Human R-Spondin 3 / RSPO3 (22-146) Protein, His Tag, 1 mg (4x 250µg)

Recombinant Human R-Spondin 3 / RSPO3 (22-146) Protein, AA Gln 22 - Val 146, expressed from human 293 cells (HEK293), His Tag

Synonym: Recombinant, human, protein, RSPO3, CRISTIN1, PWTSR, THSD2

More details

RS6-H5222-1K

Availability: within 7 days

1 800,00 €

Background
R-Spondin 3 (RSPO3) is also called cysteine-rich and single thrombospondin domain containing-1 (CRISTIN1), Protein with TSP type-1 repeat (PWTSR), is a member of the R-spondin protein family. R-spondins (RSPO) are a recently discovered secretory protein family with four members in human and mouse. Although all four RSPO proteins activate the canonical Wnt pathway, RSPO2 and RSPO3 are more potent than RSPO1, whereas RSPO4 is relatively inactive. RSPO-3 is expressed ubiquitously and expressed at higher level in placenta, small intestine, fetal thymus and lymph node. RSPO3 is the activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO3 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. RSPO3 also acts as a ligand for frizzled FZD8 and LRP6 and may negatively regulate the TGF-beta pathway.

Source
Recombinant Human R-Spondin 3 protein, (22-146) His Tag (RS6-H5222) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Val 146 (Accession # AAH22367).
Predicted N-terminus: Gln 22

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 15.3 kDa. The protein migrates as 18 kDa and 24 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

References

(1)  Chen J.-Z., et al., 2002, Mol. Biol. Rep. 29:287-292.
(2)  Kim KA,, et al., 2006, Cell Cycle 5 (1): 23–6.
(3)  Kazanskaya O., 2004, Dev Cell 7 (4): 525–34.