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Recombinant Human IL-7 R alpha / CD127 Protein, Mouse IgG2a Fc Tag, 2x 500 µg  

Recombinant Human IL-7 R alpha / CD127 Protein, Mouse IgG2a Fc Tag, 2x 500 µg

Recombinant Human IL-7 R alpha / CD127 Protein, AA Glu 21 - Gly 236, expressed from human 293 cells (HEK293), Mouse IgG2a Fc Tag

Synonym: Recombinant, human, protein, IL7Ra, CD127

More details

IL7-H5258-1K

Availability: within 7 days

2 100,00 €

Background
Interleukin-7 receptor subunit alpha (IL7Ra) is also known as CD antigen CD127, which belongs to the type I cytokine receptor family and type 4 subfamily. IL7Ra /CD127 contains 1 fibronectin type-III domain. IL7Ra /CD127 is expressed on various cell types, including naive and memory T cells and many others. IL7Ra /CD127 is receptor for interleukin-7 and also acts as a receptor for thymic stromal lymphopoietin (TSLP). The IL7 receptor is a heterodimer of IL7R and IL2RG. The TSLP receptor is a heterodimer of CRLF2 and IL7R.

Source
Recombinant Human IL-7 R alpha protein, Mouse IgG2a Fc Tag (IL7-H5258) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Gly 236 (Accession # P16871-1).
Predicted N-terminus: Glu 21

Molecular Characterization
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 52.0 kDa. The protein migrates as 65-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity-ELISA
Immobilized ActiveMax® Human IL-7, Tag Free (Cat. No. IL7-H4219) at 2 μg/mL (100 μL/well) can bind Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. No. IL7-H5258) with a linear range of 4-31 ng/mL (QC tested).

Biotinylated Human TSLP (R127A, R130A), His,Avitag (Cat. No. TSP-H82E0) immobilized at 2 μg/mL (100 μL/well) via precoated 5 μg/mL (100 μL/well) of Human TSLP R, Fc Tag (Cat. No. TSR-H525a), can bind can bind Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. No. IL7-H5258) with a linear range of 1-20 ng/mL (Routinely tested).

Bioactivity-SPR
Captured Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. No. IL7-H5258) on CM5 chip via anti-mouse antibodies surface can bind ActiveMax® Human IL-7, Tag Free (Cat. No. IL7-H4219) with an affinity constant of 13.3 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

References

(1)  Noguchi M., et al., 1994, Science 262 (5141): 1877–80.
(2)  Kroemer RT., et al., 1997, Protein Eng. 9 (12): 1135–42.
(3)  McElroy C.A., et al., 2009, Structure 17:54-65.