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Recombinant Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein, His Tag&Tag Free, 4x250 µg  

Recombinant Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein, His Tag&Tag Free, 4x250 µg

Recombinant Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein, Phe 31 - Val 992 (ITGAV) & Gly 22 - Asn 707 (ITGB6), expressed from human 293 cells (HEK293), His Tag&Tag Free

Synonym: Recombinant, human, protein, Integrin alpha V beta 6, ITGAV&ITGB6

More details

IT6-H52E1-1K

Availability: within 7 days

2 880,00 €

Background
Integrin alpha V beta 6 is a heterodimer of beta-6 associating with alpha-V. Integrin alpha-V beta-6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. Also, Integrin alpha-V beta-6 acts as a receptor for coxsackievirus A9 and coxsackievirus B1 as well as herpes simplex virus-1/HHV-1. Furthermore, it binds the TGF-beta latency‑associated peptide (LAP) and activates TGF-beta 1 or TGF-beta 3 from large latent complexes. This activation requires interaction with LTBP-1 and fibronectin, and is enhanced by PAR-1.

Source
Recombinant Human ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free (IT6-H52E1) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gly 22 - Asn 707 (ITGB6) (Accession # NP_002201 (ITGAV)& AAI21179 (ITGB6)).

Molecular Characterization
Human ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA2 and ITGB1, has a calculated MW of 127.6 kDa (ITGA2) and 83.7 kDa (ITGB1). Subunit ITGA2 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 130 kDa (ITGA2) and 100-120 kDa (ITGB1) respectively due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution

Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity-ELISA
Immobilized Human ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT6-H52E1) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Latent TGF-Beta 1, His,Avitag (Cat. No. TG1-H82Qb) with a linear range of 0.5-4 ng/mL (QC tested).

Immobilized Human Fibronectin at 10 μg/mL (100 μL/well) can bind Human ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT6-H52E1) with a linear range of 0.313-2.5 μg/mL (Routinely tested).

References

(1) Sheppard D., et al.,1990, J. Biol. Chem. 265:11502-11507.
(2) Ramsay A.G., et al., 2007, Cancer Res. 67:5275-5284.