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Recombinant Biotinylated Human ErbB3 / Her3, ultra sensitivity (primary amine labeling), 200 µg  

Recombinant Biotinylated Human ErbB3 / Her3, ultra sensitivity (primary amine labeling), 200 µg

MABSol® Recombinant Biotinylated Human ErbB3 / Her3 Protein, expressed from human HEK293 cells, His Tag, ultra sensitivity (primary amine labeling)

Synonyms: recombinant, human, biotinylated, protein, ERBB3,HER3,c-erbB3,erbB3-S,p180-ErbB3,p45-sErbB3,p85-sErbB3

More details

ER3-H8223-200

1 250,00 €

Background: ErbB3, also known as Her3 (human epidermal growth factor receptor 3), is a member of the epidermal growth factor receptor(EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain. It therefore can bind the ligand but cannot mediate the intracellular signal transduction through protein phosphorylation. However, it does form heterodimers with ErbB2 or other EGFR members responsible for tyrosine phosphorylation to give a receptor complex and initiate the related pathway, which lead to cell proliferation or differentiation. Overexpression of this protein has been reported in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

The primary amine of rhErbB3 was labeled by biotin without decreasing its bioactivity, and has been used successfully for
easy detection in ELISA, dot blot or Western blot, immunohistochemistry applications using streptavidin or avidin-conjugated
probes. Biotin and other reactive related chemicals have been removed thoroughly by desalting after reaction termination.

Source
MABSol® Recombinant Biotinylated Human ErbB3, His Tag, primary amine labeling (ER3-H8223) is expressed from human HEK293 cells. It contains AA Ser 20 - Thr 643 (Accession # NP_001973.2). It is the biotinylated form of Human ErbB3, His Tag (ER3-H5223).
Predicted N-terminus: Ser 20

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 69.5 kDa. The protein migrates as 85-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by reduced SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

References

(1) Singer, E. etal., 2001, J. Biol. Chem. 276 (47): 44266–74
(2) Kraus,M.H.etal.,1989,Proc.Natl.Acad.Sci.86:9193-9197.
(3) Carraway,K.L.etal.,1994,J.Biol.Chem.269:14303-14306.
(4) Wallasch,C.etal.,1995,EMBO.J.14:4267-4275.
(5) Alimandi,M.etal.,1995,Oncogene.10:1813-1821.
(6) Gamett,D.C.etal.,1995,J.Biol.Chem.270:19022-19027.