Products

Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Biotinylated Protein L, ultra sensitivity (primary amine labeling, long spacer), 500µg  

Recombinant Biotinylated Protein L, ultra sensitivity (primary amine labeling, long spacer), 500µg

Biotinylated Recombinant Protein L Protein, His Tag, ultra sensitivity (primary amine labeling, long spacer)

Synonym
recombinant, biotinylated, protein, RPL,Protein L

More details

RPL-P814R-500

Availability: within 7 days

96,00 €

Background
Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig(IgG,IgM,IgA,IgE and IgD) through L chain interaction, from which the name was suggested. Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., k chain of the VL domain). In humans and mice, kappa (k) light chains predominate. The remaining immunoglobulins have lambda (l) light chains. The recombinant protein contains four immunoglobulin (Ig) binding domains (Bdomains) of the native protein. Besides antibody, protein L is also suitable for binding of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).

Source
Biotinylated Recombinant Protein L, His Tag, primary amine labeling, long spacer (RPL-P814R) is expressed from E.coli cells. It is the biotinylated form of Recombinant Protein L, His Tag (RPL-P3141).

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus. The protein migrates as 50-60 kDa on a SDS-PAGE gel under reducing (R) condition. This protein can bind to VL-Kappa.

Biotinylation
The primaryy amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A specially optimized long-spacer biotin reagent (32.5 angstroms) is used in this product to minimize potential steric hindrance.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Analysis of protein association networks regulating the neuroactive metabolites production in Lactobacillus species"
Giri, Sharma
Enzyme Microb Technol (2021) 154, 109978
(2) "Preliminary Investigation of Microbiome and Dietary Differences in Patients with Phenylketonuria on Enzyme Substitution Therapy Compared to Traditional Therapies"
McWhorter, Dhillon, Hoffman
J Acad Nutr Diet (2021)
(3) "Effect of temperature on the toxicokinetics and gene expression of the pacific cupped oyster Crassostrea gigas exposed to cadmium"
Sun, Hu, Wang et al
Comp Biochem Physiol C Toxicol Pharmacol (2021)
Showing 1-3 of 489332 papers.