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Recombinant Biotinylated Human B7-H4, ultra sensitivity (primary amine labeling), 200µg  

Recombinant Biotinylated Human B7-H4, ultra sensitivity (primary amine labeling), 200µg

MABSol® Recombinant Biotinylated Human B7-H4 Protein, expressed from human HEK293 cells, His Tag, ultra sensitivity (primary amine labeling)

Synonym
recombinant, human, biotinylated, protein, B7-H4,VTCN1,B7S1,B7h.5

More details

B74-H8222-200

Availability: within 7 days

1 250,00 €

Background
V-set domain-containing T-cell activation inhibitor 1 (VTCN1) is also known as Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7H4, which belongs to the immunoglobulin superfamily and BTN/MOG family. VTCN1 contains two Ig-like V-type (immunoglobulin-like) domains. The expression of VTCN1 is up-regulated by IL6 and IL10 and is inhibited by GM-CSF and IL4 on antigen-presenting cells (APCs). VTCN1 / B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. VTCN1 involved in promoting epithelial cell transformation.

Source
MABSol® Biotinylated Human B7-H4, His Tag, primary amine labeling (B74-H8222) is expressed from human HEK293 cells. It contains AA Phe 29 - Ala 258 (Accession # NP_078902). It is the biotinylated form of Human B7-H4, His Tag (Cat. No. B74-H5222).
Predicted N-terminus: Phe 29

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.1 kDa.
The protein migrates as 43-60 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Biotinylation
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4) with Trehalose as protectant.
Contact us for customized product form or formulation.

Reconstitution
Reconstitute at 100 μg/mL in sterile deionized water. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.

Bioactivity-ELISA
Immobilized Anti-Human B7-H4 MAb at 2 μg/mL (100 μL/well) can bind Biotinylated Human B7-H4, His Tag, primary amine labeling (Cat. No. B74-H8222) with a linear range of 1-8 ng/mL (QC tested).

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

References

(1) Salceda S., et al., 2005, Exp. Cell Res. 306:128-141.
(2) Kryczek I., et al., 2006, J. Exp. Med. 203:871-881.
(3) Miyatake T., et al., 2007, Gynecol. Oncol. 106:119-127.