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Recombinant Biotinylated Human CD3 epsilon, ultra sensitivity (primary amine labeling), 200 µg  

Recombinant Biotinylated Human CD3 epsilon, ultra sensitivity (primary amine labeling), 200 µg

Biotinylated Human CD3 epsilon Protein, AA Asp 23 - Asp 126, expressed from human HEK293 cells, His Tag, ultra sensitivity (primary amine labeling)

Synonym
recombinant, human, protein, T3E,TCRE,CD3E,CD3-epsilon

More details

CDE-H8223-200

Availability: within 7 days

1 518,00 €

Background
CD3e molecule, epsilon also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.

Source
Recombinant MABSol® Biotinylated Human CD3E, His Tag, primary amine labeling (CDE-H8223) is expressed from human HEK293 cells. It contains AA Asp 23 - Asp 126 (Accession # NP_000724.1). It is the biotinylated form of Human CD3 epsilon Protein (Cat # CDE-H5223). Predicted N-terminus: Asp 23

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 12.6 kDa. The protein migrates as 15-16 kDa under reducing (R) condition (SDS-PAGE).

Biotinylation
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Biotin:protein Ratio
The biotin to protein ratio is 2-3 as determined by the HABA assay.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by reduced SDS-PAGE.

Formulation
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4) with Trehalose as protectant.

Reconstitution
Reconstitute at 100 μg/mL in sterile deionized water. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

References

(1) Gold DP, et al., 1986, Nature 321 (6068): 431–4.
(2) Clevers HC, et al., 1988, Proc. Natl. Acad. Sci. U.S.A. 85 (21): 8156–60.
(3) de Saint Basile G, et al., 2004, J. Clin. Invest. 114 (10): 1512–7.
(4) Wong S, et al., 1991, Clin. Exp. Immunol. 83 (1): 69–73.