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Recombinant Biotinylated Human HVEM / TNFRSF14, Fc Tag, Avi Tag (Avitag™), 25 µg  

Recombinant Biotinylated Human HVEM / TNFRSF14, Fc Tag, Avi Tag (Avitag™), 25 µg

MABSol® Biotinylated Human HVEM / TNFRSF14 Protein, expressed from human HEK293 cells, Fc-Tag, Avi tag (Avitag™)

Synonym:recombinant, human, biotinylated, protein, TNFRSF14,ATAR,HVEA,HVEM,LIGHTR,TR2,CD270

More details

HV4-H82F1-25

420,00 €

Background
Herpesvirus entry mediator (HVEM) is also known as TNFRSF14, TR2 (TNF receptorlike molecule) and ATAR (another TRAF associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily. HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D (gD). Two TNF superfamily ligands, including the secreted TNFβ (lymphotoxin α) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM. Besides HVEM, LIGHT can also interact with LTβR, the receptor for lymphotoxin αβ heterotrimer. The role of the HVEM LIGHT /LTβ receptor ligand pair in immune function and herpesvirus pathobiology remains to be elucidated.

Source
Biotinylated Human HVEM, Fc,Avitag (HV4-H82F1) is expressed from human 293 cells (HEK293). It contains AA Leu 39 - Val 202 (Accession # NP_003811).
Predicted N-terminus: Leu 39

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.

Molecular Characterization
This protein carries a human IgG1 Fc fragment at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 45.8 kDa. The protein migrates as 55-66 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Reconstitute at 250 μg/mL in sterile deionized water. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.

Bioactivity
Measured by its binding ability in a functional ELISA against immobilized ActiveMax® Human TNF-beta (Cat# TNB-H5214, 1 μg/well). The linear range is 0.6-10 μg/mL.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Limited Use License
The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrosbiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

References

(1) Hsu H., et al., 1997, J. Biol. Chem., 272:13471.
(2) Mauri, D.N. et al., 1998, Immunity 8:21.
(3) Montgomery, R.I. et al., 1996, Cell 87:427.
(4) Cheung,T.C.et al., 2005, Proc.Natl.Acad.Sci.USA102:13218-23.