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Recombinant Biotinylated Human IL-6, epitope tag free, ultra sensitivity (primary amine labeling), 200µg  

Recombinant Biotinylated Human IL-6, epitope tag free, ultra sensitivity (primary amine labeling), 200µg

MABSol® Recombinant Biotinylated Human IL-6, expressed from human HEK293 cells, epitope tag free, ultra sensitivity (primary amine labeling)

Synonym
recombinant, biotinylated, human, IL6,HGF,Interleukin-6,BSF2,HSF,IFNB2

More details

IL6-H8218-200

Availability: within 7 days

1 250,00 €

Background
Interleukin 6 (IL-6) is also known as HGF, BSF2,HSF, IFNB2 and IL-6, originally identified as a B cell differentiation factor, is a multifunctional cytokine that regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions.It is secreted by T cells, macrophages , monocytes, fibroblasts,endothelial cells,et.al. to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein. IL-6 is relevant to many disease processes such as diabetes,atherosclerosis, depression,Alzheimer's Disease,systemic,lupus erythematosus,prostate cancer and rheumatoid arthritis. Advanced/metastatic cancer patients have higher levels of IL-6 in their blood.Hence there is an interest in developing anti-IL-6 agents as therapy against many of these diseases.

Source
MABSol® Biotinylated Human IL-6, epitope tag free, primary amine labeling (IL6-H8218) is expressed from human HEK293 cells. It contains AA Val 30 - Met 212 (Accession # NP_000591). It is the biotinylated form of ActiveMax® Human IL-6, Tag Free (IL6-H4218).
Predicted N-terminus: Val 30

Molecular Characterization
The product does NOT contain any epitope tags.
The protein has a calculated MW of 20.8 kDa. The protein migrates as 24-27 kDa on a SDS-PAGE gel under reducing (R) condition due to different glycosylation.

Biotinylation
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4) with Trehalose as protectant.
Contact us for customized product form or formulation.

Reconstitution
Reconstitute at 100 μg/mL in sterile deionized water. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Bioactivity-ELISA
Immobilized Human IL-6 R alpha, Fc Tag (Cat. No. ILR-H5259) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-6, epitope tag free, primary amine labeling (Cat. No. IL6-H8218) with a linear range of 1-20 ng/mL (QC tested).

Bioactivity-Cell based assay
Biotinylated Human IL-6, epitope tag free, primary amine labeling (Cat. No. IL6-H8218) stimulates proliferation of TF-1 human erythroleukemic cell line. The EC50 for this effect is 0.2532-0.4489 ng/mL (Routinely tested).

References

(1) Ferguson-Smith AC, et.al. 1988, Genomics, 2 (3): 203–208.
(2) Schwantner A,et. al. 2004, J. Biol. Chem. 279 (1): 571–6.
(3) Schuster B,et. al. 2003, J. Biol. Chem. 278 (11): 9528–35.
(4) Taga T,et al.1989, Cell 58 (3): 573–81.