Products

Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Biotinylated Protein L, ultra sensitivity (primary amine labeling, long spacer), 2mg  

Recombinant Biotinylated Protein L, ultra sensitivity (primary amine labeling, long spacer), 2mg

MABSol® Biotinylated Recombinant Protein L Protein, His Tag, ultra sensitivity (primary amine labeling, long spacer)

Synonym
recombinant, biotinylated, protein, RPL,Protein L

More details

RPL-P814R-2K

Availability: within 7 days

264,00 €

Background
Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig(IgG,IgM,IgA,IgE and IgD) through L chain interaction, from which the name was suggested. Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., k chain of the VL domain). In humans and mice, kappa (k) light chains predominate. The remaining immunoglobulins have lambda (l) light chains. The recombinant protein contains four immunoglobulin (Ig) binding domains (Bdomains) of the native protein. Besides antibody, protein L is also suitable for binding of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).

Source
Biotinylated Recombinant Protein L, His Tag, primary amine labeling, long spacer (RPL-P814R) is expressed from E.coli cells. It is the biotinylated form of Recombinant Protein L, His Tag (RPL-P3141).

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus. The protein migrates as 50-60 kDa on a SDS-PAGE gel under reducing (R) condition. This protein can bind to VL-Kappa.

Biotinylation
The primaryy amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A specially optimized long-spacer biotin reagent (32.5 angstroms) is used in this product to minimize potential steric hindrance.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by reduced SDS-PAGE.

Formulation
Lyophilized from 0.22µm filtered solution in PBS (PH 7.4) with Trehalose as protectant.
Contact us for customized product form or formulation.

Reconstitution
Reconstitute at 1000 μg/mL in sterile deionized water. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Immobilized Anti-Human CTLA4 MAb at 5 μg/mL (100 μL/well)can bind Biotinylated Recombinant Protein L, His Tag, primary amine labeling, long spacer (Cat. No. RPL-P814R) with a linear range of 0.1-1 ng/mL (QC tested).
FACS Analysis of Anti-BCMA CAR
Expression293 cells were transfected with anti-BCMA-scFv and RFP tag. 2e5 of the cells were first stained with Biotinylated Recombinant Protein L, Superior Sensitivity (Cat. No. RPL-P814R, 20 µg/ml), followed by FITC Streptavidin. FITC Streptavidin was used as negative control.

References
(1) L Bjorck, 1988, The Journal of Immunology, Vol 140, Issue 4: 1194–119
(2) Nilson, B.H., et. al., 1993, J. Immunol. Methods164, 33-40.