Recombinant Biotinylated Human TFPI, ultra sensitivity (primary amine labeling), His-Tag, 25µg

Background
Tissue factor pathway inhibitor (TFPI) is also known as Extrinsic pathway inhibitor (EPI), Lipoprotein - associated coagulation inhibitor (LACI), is a plasma proteinase inhibitor synthesized by vascular endothelial cells and part of it is associated with glycosaminoglycans of these cells. TFPI is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). TFPI is a secreted protein with a N­terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C­terminal basic region. The first K domain inhibits coagulation factor VIIa complexed to tissue factor (TF); The second K domain inhibits factor Xa; The third K domain binds to heparin; The C­terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces.

Source
MABSol® Recombinant Biotinylated Human TFPI, His Tag, primary amine labeling (TFI-H8226) is expressed from human HEK293 cells. It contains AA Asp 29 - Lys 282 (Accession # NP_006278.1). It is the biotinylated form of Human TFPI, His Tag (TFI-H5226).
Predicted N-terminus: Asp 29

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 30.0 kDa. The protein migrates as 41-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Role of tissue factor pathway inhibitor in hormone-induced venous thromboembolism"
Arafat, Gennari, Ignatov et al
Blood Coagul Fibrinolysis (2023)
(2) "High risk oral contraceptive hormones do not directly enhance endothelial cell procoagulant activity in vitro"
Bouck, Arvanitis, Osburn et al
PLoS One (2023) 18 (4), e0284333
(3) "FTO overexpression expedites wound healing and alleviates depression in burn rats through facilitating keratinocyte migration and angiogenesis via mediating TFPI-2 demethylation"
Xu, Zhu, Mu et al
Mol Cell Biochem (2023)
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