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Recombinant Biotinylated Human Transferrin R/CD71, ultra sensitivity (primary amine labeling), His-Tag, 200µg  

Recombinant Biotinylated Human Transferrin R/CD71, ultra sensitivity (primary amine labeling), His-Tag, 200µg

MABSol® Recombinant Biotinylated Human Transferrin R/CD71 Protein (TFR-H8243), expressed from human HEK293 cells, His-Tag, ultra sensitivity (primary amine labeling)

Synonym
recombinant, human, biotinylated, protein, CD71,TFRC,TFR,sTfR,TfR1,Trfr

More details

TFR-H8243-200

Availability: within 7 days

1 250,00 €

Background
CD antigen CD71 is also known as Transferrin receptor protein 1, TfR, sTfR, p90, TfR1, Trfr, which belongs to the peptidase M28 family and M28B subfamily. CD71 /TFR contains one PA (protease associated) domain. CD71 / TfR1 is required for iron delivery from transferrin to cells. CD71 is a potential new target in cases of human leukomia & lymphoma. CD71 /TFRC / TfR has been shown to interact with GABARAP and HFE.

Source
Biotinylated Human Transferrin R, His Tag, primary amine labeling is expressed from human HEK293 cells. It contains AA Cys 89 - Phe 760 (Accession # AAH01188.1). It is the biotinylated form of Human Transferrin R protein, His Tag (Cat. No. CD1-H5243).
Predicted N-terminus: His

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 76.0 kDa. The protein migrates as 75-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Biotin:Protein Ratio
The biotin to protein ratio is 1-2 as determined by the HABA assay.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>92% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4) with Trehalose as protectant.
Contact us for customized product form or formulation.

Reconstitution
Reconstitute at 100 μg/mL in sterile deionized water. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Bioactivity-ELISA
Immobilized Human Transferrin, His Tag (Cat. No. TRN-H4229) at 2 μg/mL (100 μL/well) can bind Biotinylated Human Transferrin R, His Tag, primary amine labeling (Cat. No. TFR-H8243) with a linear range of 5-156 ng/mL (QC tested).

References

(1) Aisen P., 2004, Int. J. Biochem. Cell Biol. 36 (11): 2137–43.
(2) Moos T., 2002, Danish Medical Bulletin 49 (4): 279–301.