Products

Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Biotinylated Human TNF-alpha Protein, epitope tag free, ultra sensitivity (active trimer), 200 µg  

Recombinant Biotinylated Human TNF-alpha Protein, epitope tag free, ultra sensitivity (active trimer), 200 µg

MABSol® Recombinant Biotinylated Human TNF-alpha Protein, epitope tag free, ultra sensitivity (primary amine labeling) (active trimer) (MALS verified), expressed from human HEK293 cells

Synonyms: recombinant, human, biotinylated, protein, DIF, TNF-alpha, TNFA, TNFSF2, cachexin, cachectin, TNFα

More details

TNA-H8211-200

1 375,00 €

Background
Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.

Source
Recombinant MABSol® Biotinylated Human TNF-alpha, epitope tag free, primary amine labeling (active trimer) (MALS verified) (TNA-H8211) is expressed from human HEK293 cells. It contains AA Val 77 - Leu 233 (Accession # NP_000585.2). It is the biotinylated form of ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (TNA-H4211).
Predicted N-terminus: Val 77

Molecular Characterization
The product does NOT contain any epitope tags.
The protein has a calculated MW of 17.4 kDa. The protein migrates as 18 kDa on a SDS-PAGE gel under reducing (R) condition.

Biotinylation
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Biotin:Protein Ratio
The biotin to protein ratio is 1-3 as determined by the HABA assay.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by reduced SDS-PAGE.
>95% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

References
(1) Dowlati Y, Herrmann N, et al., 2010, Biol Psychiatry 67 (5): 446–457.
(2) Swardfager W, Lanctot K, et al., 2010, Biol Psychiatry 68 (10): 930–941.
(3) Locksley RM, Killeen N et al., 2001, Cell 104 (4): 487–501.