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Recombinant Biotinylated Human CD3E & CD3D Heterodimer Protein Fc Chimera, 200 µg  

Recombinant Biotinylated Human CD3E & CD3D Heterodimer Protein Fc Chimera, 200 µg

MABSol® Recombinant Biotinylated Human CD3E&CD3D Heterodimer Protein, AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D), expressed from human HEK293 cells, Fc,His Tag&Fc,Flag Tag, ultra sensitivity (primary amine labeling)

Synonyms: recombinant, human, protein, CD3 delta & CD3 epsilon,CD3 delta / epsilon

More details

CDD-H82W0-200

1 250,00 €

Background
T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.The primary amine of Human CD3E & CD3D heterodimer Fc Chimera was labeled by biotin without decreasing its bioactivity, and has been used successfully for easy detection in ELISA, dot blot or Western blot, immunohistochemistry applications using streptavidin or avidin-conjugated probes. Biotin and other reactive related chemicals have been removed thoroughly by desalting after reaction termination.

Source
MABSol® Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag, primary amine labeling (CDD-H82W0) is expressed from human HEK293 cells. It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # NP_000724.1 (CD3E) & NP_000723.1 (CD3D)). It is the biotinylated form of Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (CDD-H52W0).
Predicted N-terminus: Asp 23 (CD3E) & Phe 22 (CD3D)

Molecular Characterization
The subunit CD3E carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The subunit CD3D carries a human IgG1 Fc fragment at the C-terminus, followed by a flag tag. The protein has a calculated MW of 39.2 kDa (CD3E) and 37.5 kDa (CD3D) . The protein migrates as 45-55 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Biotinylation
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

The biotin to protein ratio is 2.5-4.5 as determined by the HABA assay.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4) with Trehalose as protectant.
Contact us for customized product form or formulation.

Reconstitution
Reconstitute at 100 μg/mL in sterile deionized water. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70° for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

References 

(1) Gold DP, et al., 1986, Nature 321 (6068): 431–4.
(2) Clevers HC, et al., 1988, Proc. Natl. Acad. Sci. U.S.A. 85 (21): 8156–60.
(3) Gil J, et al., 2011, J Clin Invest, 121(10):3872-6.
(4) Lu X, et al.,2011, Hematology, 16(3):185-9.