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Recombinant Biotinylated Human IgG1 Fc, Avi Tag (Avitag™), His Tag, 25 µg  

Recombinant Biotinylated Human IgG1 Fc, Avi Tag (Avitag™), His Tag, 25 µg

Biotinylated polyhistidine tagged human IgG1 Fc, Pro 100 - Lys 330, expressed from human HEK293 cells, Avi tag (Avitag™)

Synonyms: recombinant, human, protein, biotinylated, IgG Fc

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420,00 €

Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.

MABSol® Biotinylated Human IgG1 Fc, Avitag,His Tag (IG1-H82E2) is expressed from human HEK293 cells. It contains AA Pro 100 - Lys 330 (Accession # P01857 (C103S)).

Molecular Characterization
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 28.8 kDa. As a result of glycosylation, the protein migrates as 36 kDa under reducing (R) condition, and 60-66 kDa under non-reducing (NR) condition (SDS-PAGE).

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Less than 1.0 EU per μg by the LAL method.

>95% as determined by SDS-PAGE.

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitute at 200 μg/mL in sterile deionized water. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Immobilized Recombinant Protein A at 2 μg/mL (100 μL/well) can bind Biotinylated Human IgG1 Fc, Avitag,His Tag (Cat. No. IG1-H82E2) with a linear range of 0.3-13 ng/mL (QC tested).

Immobilized Mouse CD16-2, His Tag (Cat. No. FC4-M52H3) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IgG1 Fc, Avitag,His Tag (Cat. No. IG1-H82E2) with a linear range of 0.01-0.313 μg/mL (Routinely tested).

Limited Use License
The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrosbiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.
Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

(1) Anthony RM, et al., 2010, J Clin Immunol, 30 Suppl 1:S9-14.