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Recombinant Biotinylated Human CD27 / TNFRSF7 Protein, Fc Tag, Avi Tag (Avitag™), 200µg  

Recombinant Biotinylated Human CD27 / TNFRSF7 Protein, Fc Tag, Avi Tag (Avitag™), 200µg

Biotinylated Human CD27 / TNFRSF7 Protein, (Thr 21 - Ile 192), expressed from human 293 cells (HEK293), Fc tag, Avi tag (Avitag™)

Synonym
recombinant, human, biotinylated, protein, CD27,TNFRSF7,S152, T14,Tp55

More details

TN7-H82F6-200

Availability: within 7 days

1 380,00 €

Background
CD Antigen CD27 is also known as Tumor necrosis factor receptor superfamily member 7 (TNFRSF7), which belongs to TNF-receptor superfamily. CD27 / TNFRSF7 is found in most T-lymphocytes. CD27 / TNFRSF7 is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. CD27 has been shown to interact with SIVA1, TRAF2 and TRAF3.

Source
Biotinylated Human CD27, Fc,Avitag (TN7-H82F6) is expressed from human 293 cells (HEK293). It contains AA Thr 21 - Ile 192 (Accession # NP_001233).
Predicted N-terminus: Thr 21

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).
The protein has a calculated MW of 47.7 kDa. The protein migrates as 55-70 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Reconstitute at 200 μg/mL in sterile deionized water.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
 
Bioactivity (ELISA bioactivity)
Immobilized Human CD27 Ligand, Fc Tag (Cat. No. TN7-H526x) at 10 μg/mL (100 μL/well) can bind Biotinylated Human CD27, Fc,Avitag (Cat. No. TN7-H82F6) with a linear range of 5-156 ng/mL (QC tested).

References

(1) Zhang Z., et al., 2004, Protein Sci. 13:2819-2824.
(2) Gravestein L.A., et al., 1998, Eur. J. Immunol. 28:2208-2216.
(3) Prasad K.V.S., et al., 1997, Proc. Natl. Acad. Sci. U.S.A. 94:6346-6351.

Limited Use License
The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrosbiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.
Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.