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Recombinant Biotinylated Human IL-23 alpha & IL-12 beta, Avi Tag (Avitag™), & Tag free, 200µg  

Recombinant Biotinylated Human IL-23 alpha & IL-12 beta, Avi Tag (Avitag™), & Tag free, 200µg

Recombinant Biotinylated Human IL-23 alpha & IL-12 beta Heterodimer, Arg 20 - Pro 189 (IL23A) & Ile 23 - Ser 328 (IL12B), expressed from human 293 cells (HEK293), Avi Tag (Avitag™), His tag, & Tag Free

Synonym: recombinant, human, biotinylated, protein, IL-23 alpha & IL-12 beta

More details

ILB-H82W6-200

Availability: within 7 days

1 380,00 €

Background
Interleukin-23 subunit alpha (IL-23 alpha) can associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

Source
Recombinant Biotinylated Human IL-23A&IL-12B Heterodimer Protein, His,Avitag&Tag Free (ILB-H82W6) is expressed from human 293 cells (HEK293). It contains AA Arg 20 - Pro 189 (IL23A) & Ile 23 - Ser 328 (IL12B) (Accession # Q9NPF7-1 (IL23A) & P29460-1 (IL12B)).
Predicted N-terminus: His (IL23A) & Ile 23 (IL12B)

Molecular Characterization
Biotinylated Human IL-23A&IL-12B Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of IL23A and IL12B, has a calculated MW of 21.9 kDa (IL23A) & 34.7 kDa (IL12B). Subunit IL23A is fused with an Avi tag (Avitag™), followed by a polyhistidine tag at the N-terminus and subunit IL12B contains no tag. The predicted N-terminus is His (IL23A) & Ile 23 (IL12B). The reducing (R) protein migrates as 26 kDa (IL23A) & 40-45 kDa (IL12B) respectively due to glycosylation.

Biotin:Protein Ratio
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

References

(1) Oppmann B., et al., 2000, Immunity 13:715-725.
(2) Parham C., et al., 2002, J. Immunol. 168:5699-5708.

Limited Use License
The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrosbiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.
Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.