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Recombinant Biotinylated Human IL-4 R alpha / CD124 Protein, Avi Tag (Avitag™), His tag, 200µg  

Recombinant Biotinylated Human IL-4 R alpha / CD124 Protein, Avi Tag (Avitag™), His tag, 200µg

Biotinylated Human IL-4 R alpha / CD124 ( Met 26 - His 232), expressed from human 293 cells (HEK293), Avi tag (Avitag™), His tag (MALS verified)

Synonym
recombinant, human, biotinylated, protein, IL4R,CD124,IL4RA

More details

ILR-H82E9-200

Availability: within 7 days

1 380,00 €

Background
IL-4 R alpha is also known as IL4R, CD124, IL-4RA.This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE antibody production in B cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitis, asthma, or eczema. The binding of IL-4 or IL-13 to the IL-4 receptor on the surface of macrophages results in the alternative activation of those macrophages. Interleukin-4 receptor has been shown to interact with SHC1.

Source
Recombinant Biotinylated Human IL-4 R alpha, Avitag,His Tag (ILR-H82E9) is expressed from human 293 cells (HEK293). It contains AA Met 26 - His 232 (Accession # NP_000409.1).
Predicted N-terminus: Met 26

Molecular Characterization
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 26.9 kDa. The protein migrates as 35-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Endotoxin
Less than 1.0 EU per μg by the LAL method.
 
Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
 
Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Hyperbaric oxygen treatment on keloid tumor immune gene expression"
Wang, Shan, Liu et al
Chin Med J (Engl) (2021) 134 (18), 2205-2213
(2) "Neuroprotective role of epigallocatechin-3-gallate in acute glaucoma via the nuclear factor-κB signalling pathway"
Zhang, Chen, Gao et al
Exp Ther Med (2021) 22 (5), 1235
(3) "Lung function trajectory and biomarkers in the Tasmanian Longitudinal Health Study"
Bui, Agusti, Walters et al
ERJ Open Res (2021) 7 (3)
Showing 1-3 of 938 papers.
Please refer to product data sheet.

 

Limited Use License
The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrosbiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.
Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.