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Recombinant Biotinylated Cynomolgus BCMA / TNFRSF17 Protein, Fc Tag, Avi Tag (Avitag™), 200 µg  

Recombinant Biotinylated Cynomolgus BCMA / TNFRSF17 Protein, Fc Tag, Avi Tag (Avitag™), 200 µg

Recombinant Biotinylated Cynomolgus  / Rhesus macaque BCMA / TNFRSF17 Protein, AA Met 1 - Ala 53, expressed from human 293 cells (HEK293), Fc tag, Avi tag (Avitag™)

Synonym: Recombinant cynomolgus Protein, TNFRSF17,CD269,BCM,BCMA

More details

BCA-C82F4-200

Availability: within 7 days

1 380,00 €

Background
Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) is also known as B-cell maturation protein (BCMA), CD antigen CD269, which is a member of the TNF-receptor superfamily. TNFRSF17 contains one TNFR-Cys repeat. TNFRSF17 is expressed in mature B-cells, but not in T-cells or monocytes. TNFRSF17 is receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. TNFRSF17 promotes B-cell survival and plays a role in the regulation of humoral immunity. TNFRSF17 can activate NF-kappa-B and JNK.

Source
Recombinant Biotinylated Cynomolgus / Rhesus macaque BCMA, Fc,Avitag (BCA-C82F4) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 53 (Accession # G7Q0I4-1). In the region Met 1 - Ala 53, the AA sequence of Cynomolgus and Rhesus macaque BCMA are homologus.
Predicted N-terminus: Met 1

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 33.9 kDa. The protein migrates as 42-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Biotin:Protein Ratio
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
 
Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Risk factors associated with durable progression-free survival in relapsed or refractory multiple myeloma patients treated with anti-BCMA CAR-T cell therapy"
Zhang, Zhou, Zhao et al
Clin Cancer Res (2021)
(2) "Infectious complications in relapsed refractory multiple myeloma patients after BCMA Car t-cell therapy"
Kambhampati, Sheng, Huang et al
Blood Adv (2021)
(3) "Budget Impact of Belantamab Mafodotin (Belamaf) Adoption in the Treatment of Patients with Relapsed or Refractory Multiple Myeloma in the United States"
Shah, Tosh, Ambavane et al
Clinicoecon Outcomes Res (2021) 13, 789-800
Showing 1-3 of 756 papers.
Please refer to product data sheet.

 


Limited Use License
The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.
Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.