Recombinant human MK3 / MAPKAPK3, protein kinase,10 µg

Background: MAPKAPK3 / MK3 is part of the classical mammalian mitogen-activated protein kinase (MAPK) pathway and together with MAPKAPK2 /MK2 the major downstream targets of the p38MAPK. Based on structural and functional homology, the MAPKAPKs are divided in four subfamilies: ribosomal S6 kinase (RSK) with the members RSK1-4; mitogen- and stress-activated kinase (MSK) comprising MSK1 and MSK2; MAPK-interacting protein kinase (MNK) including MNK1 and MNK2, and the MAPK-activated protein kinases (MAPKAPK) MK2, MK3 and MK5. MK2, MK3 and MK5 are closely related, but MK2 and MK3 are more identical to each other than to MK5 but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. MAPKAPK3 is involved in diverse cellular processes, such as reorganization of the cytoskeleton, cell migration, cell-cycle regulation and chromatin remodelling.

Human MAPKAPK3 (MAP kinase activated protein kinase 3), untagged, recombinant and active enzyme, untagged, expressed in insect cells

Theoretical MW : 43.324 kDa (fusion proteins)
Expression system: E. coli BL21 (DE3)
Purification: GST-Affinity Chromatography followed by cleavage of GST-tag
Storage buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 20% glycerol
Protein concentration: 0.210 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSPH membrane
Specific activity : 188,000 pmol/mg min

Entrez Gene ID: 7867
UniProtKB: Q16644

Ordering information: shipped on dry ice

Product specific literature

Gaestel M. (2006) "MAPKAP kinases - MKs - two's company, three's a crowd."Nat Rev Mol Cell Biol. 7(2):120-30.

Ronkina N1, Menon MB, Schwermann J, Tiedje C, Hitti E, Kotlyarov A, Gaestel M.(2010) “MAPKAP kinases MK2 and MK3 in inflammation: complex regulation of TNF biosynthesis via expression and phosphorylation of tristetraprolin” Biochem Pharmacol. ;80(12):1915-20.

Ronkina N, Kotlyarov A, Gaestel M.(2008) “MK2 and MK3--a pair of isoenzymes?”Front Biosci. 2008 May 1;13:5511-21.