Recombinant human DAPK1, protein kinase domain, 10 µg

Background: The Death-associated protein kinase (DAPK) is a Ca2+/calmodulin (CaM) regulated Ser/Thr kinase,  which is ubiquitously expressed but frequently reduced in various human tumor cell lines and tissues. Activation occurs in response to death stimuli (TNF-a, C6-ceramide and INF-y) upon an increased Ca2+ level and dephosphorylation of an inhibitory site. DAPK1 acts as tumor supressor and mediates apoptotic events e.g. by upregulation of p53 and subsequently induction of the caspase-dependent cell death. On the other hand, depending on cell system and specific death signal, induction of DAPKs can lead to autophagic cell death with effects on the cytoskeleton.

recombinant human DAPK1, N-terminal fragment, amino acids M1-L363 (as in NCBI/Protein entry NP_004929.1), N-terminally fused to GST-HIS6-Thrombin cleavage site

Specific activity : 85,000 pmol/mg x min
Method for determination of Km value & specific activity: Filter binding assay MSPH membrane
Protein concentration: 0.680 mg/ml (Bradford method using BSA as standard protein)

Entrez Gene ID: 1612 
UniProtKB: P53355

Ordering information: shipped on dry ice

Product specific literature references:

Kimchi A.(2007) "Programmed cell death: From novel gene discovery to studies on network connectivity and emerging biomedical implications". Cytokine Growth Factor Rev. 18(5-6):435-40.

Wang WJ, Kuo JC, Ku W, Lee YR, Lin FC, Chang YL, Lin YM, Chen CH, Huang YP, Chiang MJ, Yeh SW, Wu PR, Shen CH, Wu CT, Chen RH.(2007) " The Tumor Suppressor DAPKIs Reciprocally Regulated by Tyrosine Kinase Src and Phosphatase LAR". Mol Cell. 27(5):701-16.

Bialik S, Kimchi A.(2006) " The death-associated protein kinases: structure, function, and beyond." Annu Rev Biochem. 2006;75:189-210