Recombinant human MK5 / MAPKAPK5, protein kinase,10 µg

Background: MAPKAPK5 / MK5 is together with MAPKAPK2 /MK2 and MAPKAPK3 /MK3 part of the family of MAPK-activated protein kinases (MAPKAPK) and serves as targets for MAPK. Whereas MK2 and MK3 are activated by p38 MAPK MK5 is phosphorylated by ERK3 and ERK4 making MK5 unique among the MAPKAPKs. MK5 has a unique C-terminal extension of ∼ 75 amino acids, but lacks the N-terminal Pro-rich motif present in MK2 and MK3. MK5 contains putative cAMP-dependent protein kinase (PKA) phosphoacceptor sites, and it was shown that PKA phosphorylates MK5 at Ser-115. Activated MK5 can in turn phosphorylate HSP27 and allows F-actin remodeling, affect the transcriptional activity of HSP1,  and phosphorylate Septin8 (SEPT8), a small GTPase involved in neurosecretion and neuroplasticity.

Human MAPKAPK5 (mitogen-activated protein kinase-activated protein kinase 5), full length, amino acids M1-Q473 activated, N-terminally fused to GST-HIS6-Thrombin cleavage site

Theoretical MW :  84.1 kDa (fusion proteins)
Expression system: Sf9 cells
Purification: One-step affinity purification using glutathione agarose
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol
Protein concentration: 0.113 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSPH membrane
Specific activity : 2,000 pmol/mg min

Entrez Gene ID: 8550
UniProtKB: Q81W41

Ordering information: shipped on dry ice

Product specific literature

Gaestel M. (2006) "MAPKAP kinases - MKs - two's company, three's a crowd."Nat Rev Mol Cell Biol. 7(2):120-30.

Moens U1, Kostenko S.(2013) “Structure and function of MK5/PRAK: the loner among the mitogen-activated protein kinase-activated protein kinases.” Biol Chem.394(9):1115-32.