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Recombinant human PDGFRb /CD140b Protein, His tag, 2x500 µg  

Recombinant human PDGFRb /CD140b Protein, His tag, 2x500 µg

Recombinant Human PDGF R beta / CD140b Protein, Leu 33 - Phe 530, produced in human 293 cells (HEK293), His tag

Synonym
Recombinant Human Protein, PDGFRB, PDGFR-B, CD140B, JTK12,PDGF-R-beta, PDGFR, PDGFR1, PDGFR-1, PDGFR-beta

More details

PDB-H5220-1K

Availability: within 7 days

1 400,00 €

Background
Human platelet-derived growth factor receptor, beta polypeptide (PDGFRB),also called J03278, M21616, CD140B, JTK12, PDGF-R-beta and PDGFR, is receptor that binds specifically to PDGFB and has a tyrosine-protein kinase activity.[1] Is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor (PDGF) family. The PDGFR/PDGF system includes two receptors (PDGFRA and PDGFRB) and four ligands (A, B, C and D). The receptors PDGFRA and PDGFRB are related in sequence and both are members of the class III subtype of receptor tyrosine kinases (RTKs). Other class III RTKs are CSF1R, KIT and FLT3. PDGF binding induces receptor homo- and heterodimerization and signal transduction. The expression of the α and β receptors is independently regulated in various cell types. Recombinant soluble PDGFRB binds PDGF with high affinity and is potent PDGF antagonist.[2] The ligands form either homo- or heterodimers (PDGF-AA, -AB, -BB, -CC, -DD). The four PDGFs are inactive in their monomeric forms. The PDGFs bind to the protein tyrosine kinase receptors PDGF receptor-α and -β. These two receptor isoforms dimerize upon binding the PDGF dimer, leading to three possible receptor combinations, namely -αα, -ββ and -αβ. PDGF-CC specifically interacts with PDGFR-αα and -αβ, but not with –ββ, and thereby resembles PDGF-AB. PDGF-DD binds to PDGFR-ββ with high affinity, and to PDGFR-αβ to a much lower extent and is regarded as PDGFR-ββ specific. PDGF-AA binds only to PDGFR-αα, while PDGF-BB is the only PDGF that can bind all three receptor combinations with high affinity. [3]

Source
Recombinant Human PDGF R beta / CD140b Protein (Human PDGF R beta, His Tag) Leu 33 - Phe 530 (Accession # AAH32224) was produced in human 293 cells (HEK293).

Molecular Characterization
rhPDGFRB contains C-terminal polyhistidine tag and has a calculated MW of 57.1 kDa. As a result of glycosylation, DTT-reduced protein migrates as 85-95 kDa polypeptide in SDS-PAGE.

Endotoxin
Less than 1.0 EU per μg of the rhPDGFRB by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Chi, KD. et al., 1997, Oncogene 15: 1051-7.
(2) Golub TR. et al., Cell. 1994 ; 77 (2) : 307-316.
(3) Morerio, C. et al., 2004, Cancer Res. 64: 2649-51.