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Recombinant human CD146 / MCAM Protein, His tag, 2x500 µg  

Recombinant human CD146 / MCAM Protein, His tag, 2x500 µg

Recombinant Human CD146 /MCAM Protein (Val24-Gly559), produced in human 293 cells (HEK293), His tag

Synonym
Recombinant Human Protein,MCAM, CD146, MUC18, cluster of differentiation 146

More details

CD6-H5229-1K

Availability: within 7 days

2 160,00 €

Background
CD146, also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. As a member of the Immunoglobulin superfamily, It is expressed on chicken embryonic spleen and thymus, activated human T cells, endothelial progenitors such as angioblasts and mesenchymal stem cells, and strongly expressed on blood vessel endothelium and smooth muscle. CD146 has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. The CD146+ T cells display an immunophenotype consistent with effector memory cells and have a distinct gene profile from the CD146- T cells. As a Ca2+ independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor,CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis or immune recognition [1-5].

Source
Recombinant Human CD146 /MCAM Protein (rhCD146) Val24-Gly559 (Accession # NP_006491.2) was produced in human 293 cells (HEK293)

Molecular Characterization
rhCD146, fused with a C-terminal 6×his tag and has a calculated MW of 61.3 kDa expressed. The predicted N-terminus is Val 24. Protein migrates as 80-90 kDa in reduced SDS-PAGE resulting from glycosylation.

Endotoxin
Less than 1.0 EU per µg of the rhCD146 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 µm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4C-8C); After reconstitution under sterile conditions for 1 month (4C-8C) or 3 months (-20C to -70C).

References

(1) Kuske MD, et al,1999, Cytogenet. Cell Genet. 87 (3-4): 258
(2) Elshal MF, et al, 2005, Blood. 106 (8): 2923–4
(3) Elshal MF, et al, 2007, BMC Immunol. 8: 29
(4) Covas DT, et al, 2008, Exp. Hematol. 36 (5): 642–54
(5) Russell KC, et al, 2010, Stem Cells 28 (4): 788–98