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Recombinant human CD146 / MCAM Protein, His tag, 2x500 µg  

Recombinant human CD146 / MCAM Protein, His tag, 2x500 µg

Recombinant Human CD146 /MCAM Protein (Val24-Gly559), produced in human 293 cells (HEK293), His tag

Recombinant Human Protein,MCAM, CD146, MUC18, cluster of differentiation 146

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Availability: within 7 days

2 160,00 €

CD146, also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. As a member of the Immunoglobulin superfamily, It is expressed on chicken embryonic spleen and thymus, activated human T cells, endothelial progenitors such as angioblasts and mesenchymal stem cells, and strongly expressed on blood vessel endothelium and smooth muscle. CD146 has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. The CD146+ T cells display an immunophenotype consistent with effector memory cells and have a distinct gene profile from the CD146- T cells. As a Ca2+ independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor,CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis or immune recognition [1-5].

Recombinant Human CD146 /MCAM Protein (rhCD146) Val24-Gly559 (Accession # NP_006491.2) was produced in human 293 cells (HEK293)

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 61.3 kDa. The protein migrates as 70-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Less than 1.0 EU per µg of the rhCD146 by the LAL method.

>95% as determined by SDS-PAGE.

Lyophilized from 0.22 µm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4C-8C); After reconstitution under sterile conditions for 1 month (4C-8C) or 3 months (-20C to -70C).


(1) Kuske MD, et al,1999, Cytogenet. Cell Genet. 87 (3-4): 258
(2) Elshal MF, et al, 2005, Blood. 106 (8): 2923–4
(3) Elshal MF, et al, 2007, BMC Immunol. 8: 29
(4) Covas DT, et al, 2008, Exp. Hematol. 36 (5): 642–54
(5) Russell KC, et al, 2010, Stem Cells 28 (4): 788–98