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Recombinant human CD155 Protein, His Tag, 1mg  

Recombinant human CD155 Protein, His Tag, 1mg

Recombinant Human CD155 /PVR Protein (Trp21-Asn343), produced in human 293 cells (HEK293), His Tag

Synonym
Recombinant Human Protein, PVR, PVS, CD155, TAGE4, HVED, NECL5, cluster of differentiation 155

More details

CD5-H5223-1K

Availability: within 7 days

1 800,00 €

Background
CD155 is a Type I transmembrane glycoprotein in the immunoglobulin superfamily.[1] Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates, CD155's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells.[2] CD155/PVR was originally isolated based on its ability to mediate polio virus attachment to host cells [1-3]. The fulllength (or CD155 alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20 aa signal sequence, a 323 aa extracellular region, a 24 aa TM segment and a 50 aa cytoplasmic tail. The extracellular region contains one N terminal V type and two C2 type Ig like domains [1-3]. CD155 is a transmembrane protein with 3 extracellular immunoglobulin-like domains, D1-D3, where D1 is recognized by the virus.[4] Low resolution structures of CD155 complexed with poliovirus have been obtained using electron microscopy[1] while a high resolution structures of theectodomain D1 and D2 of CD155 were solved by x-ray crystallography.[4]

Source
Recombinant Human CD155 /PVR Protein (rhCD155) Trp21-Asn343 (Accession # NP_006496.2 ) was produced in human 293 cells (HEK293)

Molecular Characterization
rhCD155, fused with a C-terminal 6×his tag and has a calculated MW of 28 kDa. The predicted N-terminus is Trp21. DTT-reduced protein migrates as 50-65 kDa polypeptide in SDS-PAGE resulting from glycosylation.

Endotoxin
Less than 1.0 EU per µg of the rhCD155 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 µm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).  

Bioactivity
Immobilized Human Mesothelin, Fc Tag (Cat. No. MSN-H526x) at 0.5 μg/mL (100 μL/well) can bind Anti-Human MSLN Mab with a linear range of 0.8-3 ng/mL (QC tested).

 

References

(1) Mendelsohn CL, Wimmer E, Racaniello VR (1989) Cell 56 (5): 855–65
(2) Maier MK, Seth S, Czeloth N, et al. (2007). European Journal of Immunology 37 (8): 2214
(3) Koike, H. et al. (1990) EMBO J. 9:3217 (4) Zhang P, Mueller S, Morais MC. Proc. Natl. Acad. Sci. U.S.A. 105 (47): 18284–9