Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant human CD146 / MCAM Protein, Fc Tag, 1mg  

Recombinant human CD146 / MCAM Protein, Fc Tag, 1mg

Recombinant Human CD146 / MCAM Protein (Val 24 - Gly 559), produced in human 293 cells (HEK293), Fc Tag

Synonym
Recombinant Human Protein,MCAM, CD146, MUC18

More details

CD6-H5253-1K

Availability: within 7 days

1 800,00 €

Background
CD146, also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. As a member of the Immunoglobulin superfamily, It is expressed on chicken embryonic spleen and thymus, activated human T cells, endothelial progenitors such as angioblasts and mesenchymal stem cells, and strongly expressed on blood vessel endothelium and smooth muscle. CD146 has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. The CD146+ T cells display an immunophenotype consistent with effector memory cells and have a distinct gene profile from the CD146- T cells. As a Ca2+ independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor,CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis or immune recognition [1-5]

Source
Recombinant Human CD146 /MCAM Protein, With C-Fc Tag (rh CD146 /MCAM Fc Chimera) Val 24 - Gly 559 (Accession # NP_006491.2) was produced in human 293 cells (HEK293).

Molecular Characterization
rh CD146 /MCAM Fc Chimera, fused with Fc fragment of human IgG1 at the C-terminus, has a calculated MW of 85.9 kDa. The predicted N-terminus is Val 24. DTT-reduced Protein migrates as 120-130 kDa due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh CD146 /MCAM Fc Chimera by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Kuske MD, et al.,1999, Cytogenet. Cell Genet. 87 (3-4): 258.
(2) Elshal MF, et al., 2005, Blood. 106 (8): 2923–4.
(3) Elshal MF, et al., 2007, BMC Immunol. 8: 29.
(4) Covas DT, et al., 2008, Exp. Hematol. 36 (5): 642–54.
(5) Russell KC, et al., 2010, Stem Cells 28 (4): 788–98.