Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant human SEMA4D / CD100 Protein, Fc Tag, 4x250 µg  

Recombinant human SEMA4D / CD100 Protein, Fc Tag, 4x250 µg

Recombinant human SEMA4D /CD100 Protein, Met 22 - Arg 734, produced in human 293 cells (HEK293), Fc Tag

Synonym
Recombinant human protein, SEMA4D, C9orf164, CD100, FLJ33485, FLJ46484, M-sema-G, SEMAJ, coll-4, Semaphorin-4D

More details

CD0-H5257-1K

Availability: within 7 days

1 600,00 €

Background
Cluster of Differentiation 100 (CD100), also known as Semaphorin-4D (SEMA4D), is a single-pass type I membrane protein which belongs to the semaphorin family, and is a human 150-kDa homodimer expressed at the surface of most hemopoietic cells.[1] Semaphorin genes encode soluble and membrane-bound proteins, most of which have been shown to act as chemorepellents on growth cone guidance. CD100 is discrete, as it is a transmembrane leukocyte surface molecule that can also exist in a soluble form.[1] Semaphorin 4D (Sema 4D) is an axon guidance molecule which is secreted by oligodendrocytes and induces growth cone collapse in the central nervous system. By binding plexin B1 receptor it functions as an R-Ras GTPase-activating protein (GAP) and repels axon growth cones in both the mature central nervous system.[2] In the immune system, CD100 binds CD72 to activate B cells and dendritic cells, though much about this interaction is still under investigation.[3-4] It is involved in oligodendrogenesis during development and during recovery from ischemic injury.

Source
Recombinant human SEMA4D /CD100 Protein, With C-Fc Tag (rhCD100-Fc) Met 22 - Arg 734 (Accession # AAH54500) was produced in human 293 cells (HEK293).

Molecular Characterization
rhCD100-Fc, fused with Fc fragment Of human IgG1 at the C-terminus and has a calculated MW of 105.4 kDa expressed. The predicted N-terminus is Met 22. Protein migrates as 120-140 kDa in reduced SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per µg of the rhCD100-Fc by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 µm filtered solution in 50 mM tris, 100 mM glycine, pH7.0 . Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Delaire S, et al., 2001, J. Immunol. 166 (7): 4348–54.
(2) Ito Y, et al., 2006, EMBO Reports 7 (7): 704–9.
(3) Kumanogoh A, et al., 2000, Immunity 13 (5): 621–31.
(4) Ishida I, et al., 2007, Int. Immunol. 15 (8): 1027–34.