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Recombinant human ICAM1 /CD54 Protein, Fc Tag, 1mg  

Recombinant human ICAM1 /CD54 Protein, Fc Tag, 1mg

Recombinant Human ICAM1 /CD54 Protein, Gln27-Glu480, produced in human HEK293 cells, Fc Tag

Synonym
Recombinant Human Protein, ICAM1, ICAM-1, BB2, BB-2, CD54, CD-54, P3.58

More details

IC1-H5250-1K

Availability: within 7 days

1 440,00 €

Background
Inter-Cellular Adhesion Molecule 1 (ICAM-1) is also known as Cluster of Differentiation 54 (CD54), is a member of the immunoglobulin superfamily, and is a cell surface glycoprotein which is typically expressed in low concentrations on endothelial cells and cells of the immune system. The protein encoded by this gene is a type of intercellular adhesion molecule continuously present in low concentrations in the membranes of leukocytes and endothelial cells. Upon cytokine stimulation, the concentrations greatly increase. ICAM-1 can be induced by interleukin-1 (IL-1) and tumor necrosis factor alpha (TNFα) and is expressed by the vascular endothelium, macrophages, and lymphocytes. ICAM-1 is a ligand for LFA-1 (integrin), a receptor found on leukocytes. When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 and then transmigrate into tissues. ICAM-1 has been implicated in subarachnoid hemorrhage (SAH). Levels of ICAM-1 are shown to be significantly elevated in patients with SAH over control subjects in many studies. ICAM-1 expressed by respiratory epithelial cells is also the binding site for rhinovirus, the causative agent of most common colds. 

Source
Recombinant Human ICAM1 /CD54 Protein, With C-Fc Tag (rhICAM1-Fc) Gln27-Glu480 (Accession # AAH15969) was produced in human HEK293 cells

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 75.7 kDa. The protein migrates as 90-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhICAM1-Fc by the LAL method.

Purity
>98% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Immobilized Human ITGALB2, His tag at 5 μg/mL (100 μL/well) can bind Human ICAM-1, Fc Tag (Cat. No. IC1-H5250) with a linear range of 0.082-1.28 μg/mL (Routinely tested).

References

(1) Rothlein, R. D., 1986, Journal of Immunology 137 (4): 1270–1274.
(2) Yang L, et al., 2005, Blood 106 (2): 584–92.
(3) Polin RS, et al., 1998, J. Neurosurg. 89 (4): 559–67.
(4) Frijns CJ, Kappelle LJ., 2002, Stroke 33 (8): 2115–22.