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Recombinant human PECAM1 /CD31 Protein, Fc Tag, 1mg  

Recombinant human PECAM1 /CD31 Protein, Fc Tag, 1mg

Recombinant human PECAM1 /CD31 Protein, Gln28-Lys601, produced in human 293 cells (HEK293), Fc Tag

Synonym
Recombinant human Protein, PECAM1, CD31, CD-31, FLJ34100, FLJ-34100, FLJ58394, FLJ-58394, PECAM-1

More details

CD1-H5258-1K

Availability: within 7 days

1 440,00 €

Background
Cluster of Differentiation 31 (CD31), also known as Platelet endothelial cell adhesion molecule (PECAM-1), is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, macrophages and Kupffer cells, granulocytes, T / NK cells, lymphocytes, megakaryocytes, osteoclasts, neutrophils, certain tumors, and is the only known member of the CAM family on platelets. CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. CD31 plays a key role in removing aged neutrophils from the body. CD31 mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development. In addition, it has been shown that CD31 expression is up-regulated by LPS stimulation, and might function as a feedback negative regulator of LPS inflammatory response in macrophages.[1-3]

Source
Recombinant human PECAM1 /CD31 Protein, With C-Fc Tag (rhCD31-Fc) Gln28-Lys601 (Accession # AAH22512.1) was produced in human 293 cells (HEK293)

Molecular Characterization
rhCD31-Fc, fused with Fc fragment of human IgG1 at the C-terminus and has a calculated MW of 90.5 kDa expressed. The predicted N-terminus is Gln28. Protein migrates as 100-140 kDa in reduced SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCD31-Fc by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Newman PJ, Newman DK., 2004, Arterioscler. Thromb. Vasc. Biol. 23 (6): 953–64.
(2) Kalinowska A, Losy J., 2007, Eur. J. Neurol. 13 (12): 1284–90.
(3) Almendro N, et al., 2007, J. Immunol. 157 (12): 5411–21.