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Recombinant human DPPIV/CD26 Protein, Fc Tag, 2x500µg  

Recombinant human DPPIV/CD26 Protein, Fc Tag, 2x500µg

Recombinant Human DPPIV /DPP4 /CD26 Protein, Asn29 - Pro766, produced in human 293 cells (HEK293), Fc Tag (active enzyme)

Synonym
Recombinant Human Protein, DPP4, ADABP, ADCP2, CD26, DPPIV, TP103

More details

DP4-H5266-1K

Availability: within 7 days

1 800,00 €

Background
Dipeptidyl peptidase-IV (DPPIV), also known as adenosine deaminase complexing protein 2, DPPIV or CD26 is antigenic enzyme expressed on the surface of most cell types and is associated with immune regulation, signal transduction and apoptosis. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides.[1] The substrates of DPPIV are proline (or alanine)-containing peptides and include growth factors, chemokines, neuropeptides, and vasoactive peptides.[2] DPPIV plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1.[3] DPPIV plays an important role in tumor biology, and is useful as a marker for various cancers, with its levels either on the cell surface or in the serum increased in some neoplasms and decreased in others.[4] DPPIV also binds the enzyme adenosine deaminase specifically and with high affinity. The significance of this interaction has yet to be established.

Source
Recombinant Human DPPIV /DPP4 /CD26 Protein, Fc Tag (DP4-H5266) is expressed from human 293 cells (HEK293). It contains AA Asn 29 - Pro 766 (Accession # NP_001926.2).

Molecular Characterization
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 112.3 kDa. The protein migrates as 90-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU endotoxin per μg rhDPPIV-Fc by the LAL method.

Purity
>95% purity as determined by SDS-PAGE of reduced rhDPPIV-Fc.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "The Role of Post-Translational Modifications of Chemokines by CD26 in Cancer"
De Zutter, Van Damme, Struyf
Cancers (Basel) (2021) 13 (17)
(2) "Fortification of Maize Tortilla with an Optimized Chickpea Hydrolysate and Its Effect on DPPIV Inhibition Capacity and Physicochemical Characteristics"
Acevedo-Martinez, Gonzalez de Mejia
Foods (2021) 10 (8)
(3) "Peripheral endomorphins drive mechanical alloknesis under the enzymatic control of CD26/DPPIV"
Komiya, Tominaga, Hatano et al
J Allergy Clin Immunol (2021)
Showing 1-3 of 665 papers.
Please refer to product data sheet.

 

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