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Recombinant human LIFR /CD118 Protein, Fc Tag, 50µg  

Recombinant human LIFR /CD118 Protein, Fc Tag, 50µg

Recombinant Human LIFR /CD118 Protein, Gln 45 - Ser 833, expressed in human 293 cells (HEK293), Fc Tag

Synonym
Recombinant Human Protein, LIFR, CD118, FLJ98106, FLJ99923, SJS2, STWS, SWS, Leukemia inhibitory factor receptor

More details

LIR-H4252-50

Availability: within 7 days

168,00 €

Background
Leukemia inhibitory factor receptor also known as LIFR; CD118; FLJ98106; FLJ99923; LIF-R; SJS2; STWS; SWS, is the receptor for leukemia inhibitory factor (LIF). The leukemia inhibitory factor is a polyfunctional cytokine that affects the differentiation, survival, and proliferation of a wide variety of cells in the adult and the embryo. LIF action appears to be mediated through a high-affinity receptor complex composed of a low-affinity LIF binding chain (LIF receptor) and a high-affinity converter subunit, gp130. Both LIFR and gp130 are members of a family of cytokine receptors that includes components of the receptors for the majority of hematopoietic cytokines and for cytokines that affect other systems, including the ciliary neurotrophic factor, growth hormone and prolactin Defects in LIFR are the cause of Stueve-Wiedemann syndrome (SWS), a severe autosomal recessive condition and belongs to the group of the bent-bone dysplasias.[1-5]

Source
Recombinant Human LIFR /CD118 Protein, With C-Fc Tag (rhLIFR-Fc) contain NP_001121143 over Gln 45 - Ser 833 positions, was expressed in human 293 cells (HEK293).

Molecular Characterization
rhLIFR-Fc, fused with the Fc region of human IgG1 at the C-terminus, The reduced monomer has a calculated molecular mass of 115.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhLIFR-Fc monomer is approximately 135-150 kDa due to the glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhLIFR-Fc by the LAL method.

Purity
>95% purity as determined by SDS-PAGE of reduced rhLIFR-Fc.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Immobilized Human LIF, Tag Free (Cat. No. LIF-H521b) at 0.2μg/mL (100 μL/well) can bind Human LIF R, Fc Tag (Cat. No. LIR-H4252) with a linear range of 7.8-31.25 ng/mL (QC tested).

References

(1) Timmermann, Andreas, et al. 2002, Eur. J. Biochem. (Germany) 269 (11): 2716–26.
(2) Gearing, D.P. et al.,1991, EMBO J. 10 (10): 2839-2848
(3) Gearing, D.P. et al.,1992, New Biol. 4 (1): 61-65
(4) Mosley, B. et al.,1996, J. Biol. Chem. 271 (51): 32635-32643
(5) Lass, A. et al.,2002, Fertil. Steril. 76 (6): 1091-1096